Isolation and characterization of an acrylamide-degrading Antarctic bacterium
The presence of acrylamide in the environment poses a threat due to its well known neurotoxic, carcinogenic and teratogenic properties. Human activities in various geographical areas are the main anthropogenic source of acrylamide pollution. In this work, an acrylamide-degrading bacterium was isol...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English English |
Published: |
Triveni Enterprises, Lucknow
2009
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Online Access: | http://psasir.upm.edu.my/id/eprint/11992/1/Journal_of_Environmental_Biology.pdf http://psasir.upm.edu.my/id/eprint/11992/ |
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Summary: | The presence of acrylamide in the environment poses a threat due to its well known neurotoxic, carcinogenic and teratogenic
properties. Human activities in various geographical areas are the main anthropogenic source of acrylamide pollution. In this work, an
acrylamide-degrading bacterium was isolated from Antarctic soil. The physiological characteristics and optimum growth conditions of the
acrylamide-degrading bacteria were investigated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ7 based on carbon
utilization profiles using Biolog GN plates and partial 16S rDNA molecular phylogeny. The results showed that the best carbon sources for
growth was glucose and sucrose with no significant difference in terms of cellular growth between the two carbon sources (p>0.05). This
was followed by fructose and maltose with fructose giving significantly higher cellular growth compared to maltose (p<0.05). Lactose and
citric acid did not support growth. The optimum acrylamide concentration as a nitrogen source for cellular growth was at 500 mgl-1. At this
concentration, bacterial growth showed a 2-day lag phase before degradation took place concomitant with an increase in cellular growth.
The isolate exhibited optimum growth in between pH 7.5 and 8.5. The effect of incubation temperature on the growth of this isolate showed
an optimum growth at 15°C. The characteristics of this isolate suggest that it would be useful in the bioremediation of acrylamide |
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