Application of a high density adsorbent in expanded bed adsorption of lipase from Burkholderia pseudomallei

• Main Authors: Yong, Hor Shee, Tey, Beng Ti, Hii, Siew Ling, Mustapa Kamal, Siti Mazlina, Ariff, Arbakariya, Ling, Tau Chuan
• Format: Article
• Language: English
• Published: Academic Journals 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/11193/1/61.pdf; http://psasir.upm.edu.my/id/eprint/11193/; http://www.academicjournals.org/journal/AJB/article-abstract/51527AC30230

The application of STREAMLINE Direct HST adsorbent in expanded bed adsorption of lipase from Burkholderia pseudomallei was explored in this study. Scouting of optimum binding and elution condition was performed in batch binding mode. Theaddition of 0.2 M salt in acetate buffer (pH 5) during adsorpti...