Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells
In this study, we synthesized, characterized, and explored the anti-microbial and anti-cancer effects of albumin–chlorogenic acid nanoparticles (NPs). Characterization studies with a UV-vis spectrophotometer, FTIR, PL spectrum, TEM, FESEM, XRD, and DLA analysis showed patterns confirming the physio–...
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my.upm.eprints.1075582024-08-29T07:39:23Z http://psasir.upm.edu.my/id/eprint/107558/ Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells Alzahrani, Badr Elderdery, Abozer Y. Alsrhani, Abdullah Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling In this study, we synthesized, characterized, and explored the anti-microbial and anti-cancer effects of albumin–chlorogenic acid nanoparticles (NPs). Characterization studies with a UV-vis spectrophotometer, FTIR, PL spectrum, TEM, FESEM, XRD, and DLA analysis showed patterns confirming the physio–chemical nature of biogenic nanocomposites. Further, anti-microbial studies using bacterial strains Staphylococcus aureus, Streptococcus pneumonia, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Vibrio cholera, and fungal strain Candida albicans showed significant (p < 0.05) anti-bacterial and anti-fungal activities. Next, we used MDA-MB-435s, a human cell line, to evaluate the anti-cancer effects of albumin–chlorogenic acid NPs. Cytotoxic studies revealed its IC50 concentration at 24 μg/mL after a 24 h treatment of MDA-MB-435s cells. We chose this IC50 dose to analyze albumin–chlorogenic acid NPs anti-cancer effects in vitro. MDA-MB-435s cells exposed to our NPs were studied via AO/EtBr staining, cell cycle analyses via PI staining, the status of whole genomic damage via comet assay, levels of apoptotic cells via annexin V/PI staining, ROS generation via DCFH-DA staining, an assay of antioxidant enzymes catalase, superoxide dismutase, and antioxidant GSH, via ELISA analyses of apoptotic markers caspase-3, 8, 9, Bax, Bcl-2, CytC, and p53, PI3/AKT/mTOR pathway. Our results collectively showed albumin–chlorogenic acid NPs induced apoptosis via p53-dependent and PI3/AKT/mTOR inhibition in MDA-MB-435s cells. Our results denote albumin–chlorogenic acid NPs can be used as an effective candidate for anti-microbial and anti-cancer applications; however, further in vivo confirmatory studies are warranted. MDPI 2023-04-22 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/107558/1/nanomaterials-13-01438.pdf Alzahrani, Badr and Elderdery, Abozer Y. and Alsrhani, Abdullah and Alzerwi, Nasser A. N. and Althobiti, Maryam Musleh and Rayzah, Musaed and Idrees, Bandar and Elkhalifa, Ahmed M. E. and Subbiah, Suresh K. and Mok, Pooi Ling (2023) Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells. Nanomaterials, 13 (9). pp. 1-17. ISSN 2079-4991 https://www.mdpi.com/2079-4991/13/9/1438 10.3390/nano13091438 |
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In this study, we synthesized, characterized, and explored the anti-microbial and anti-cancer effects of albumin–chlorogenic acid nanoparticles (NPs). Characterization studies with a UV-vis spectrophotometer, FTIR, PL spectrum, TEM, FESEM, XRD, and DLA analysis showed patterns confirming the physio–chemical nature of biogenic nanocomposites. Further, anti-microbial studies using bacterial strains Staphylococcus aureus, Streptococcus pneumonia, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Vibrio cholera, and fungal strain Candida albicans showed significant (p < 0.05) anti-bacterial and anti-fungal activities. Next, we used MDA-MB-435s, a human cell line, to evaluate the anti-cancer effects of albumin–chlorogenic acid NPs. Cytotoxic studies revealed its IC50 concentration at 24 μg/mL after a 24 h treatment of MDA-MB-435s cells. We chose this IC50 dose to analyze albumin–chlorogenic acid NPs anti-cancer effects in vitro. MDA-MB-435s cells exposed to our NPs were studied via AO/EtBr staining, cell cycle analyses via PI staining, the status of whole genomic damage via comet assay, levels of apoptotic cells via annexin V/PI staining, ROS generation via DCFH-DA staining, an assay of antioxidant enzymes catalase, superoxide dismutase, and antioxidant GSH, via ELISA analyses of apoptotic markers caspase-3, 8, 9, Bax, Bcl-2, CytC, and p53, PI3/AKT/mTOR pathway. Our results collectively showed albumin–chlorogenic acid NPs induced apoptosis via p53-dependent and PI3/AKT/mTOR inhibition in MDA-MB-435s cells. Our results denote albumin–chlorogenic acid NPs can be used as an effective candidate for anti-microbial and anti-cancer applications; however, further in vivo confirmatory studies are warranted. |
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Article |
author |
Alzahrani, Badr Elderdery, Abozer Y. Alsrhani, Abdullah Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling |
spellingShingle |
Alzahrani, Badr Elderdery, Abozer Y. Alsrhani, Abdullah Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells |
author_facet |
Alzahrani, Badr Elderdery, Abozer Y. Alsrhani, Abdullah Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling |
author_sort |
Alzahrani, Badr |
title |
Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells |
title_short |
Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells |
title_full |
Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells |
title_fullStr |
Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells |
title_full_unstemmed |
Effects of albumin–chlorogenic acid nanoparticles on apoptosis and PI3K/Akt/mTOR pathway inhibitory activity in MDA-MB-435s cells |
title_sort |
effects of albumin–chlorogenic acid nanoparticles on apoptosis and pi3k/akt/mtor pathway inhibitory activity in mda-mb-435s cells |
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MDPI |
publishDate |
2023 |
url |
http://psasir.upm.edu.my/id/eprint/107558/1/nanomaterials-13-01438.pdf http://psasir.upm.edu.my/id/eprint/107558/ https://www.mdpi.com/2079-4991/13/9/1438 |
_version_ |
1809142959552069632 |
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13.19449 |