Nanoplate-based digital PCR for highly sensitive pork DNA detection targeting multi-copy nuclear and mitochondrial genes

• Main Authors: Mokhtar, Nur Fadhilah Khairil, Shun, Ying Quah, Raja Nhari, Raja Mohd Hafidz, Mohamad, Nurhidayatul Asma, Shahidan, Nur Maisarah, Warsanah, Irwan Hanish, Mohd Hashim, Amalia
• Format: Article
• Published: Taylor and Francis 2024
• Online Access: http://psasir.upm.edu.my/id/eprint/105707/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181680757&doi=10.1080%2f19440049.2023.2298476&partnerID=40&md5=f0b824031bfa10cdeba005581aacd323

The inclusion of ingredients derived from pigs in highly processed consumer products poses a significant challenge for DNA-targeted analytical enforcement, which could be overcome by using digital PCR. However, most species detection methods use digital PCR to target single-copy nuclear genes, which...