DNA-free CRISPR/Cas9 genome editing system for oil palm protoplasts using multiple ribonucleoproteins (RNPs) complexes

• Main Authors: Norfaezah, Jamaludin, Masani, Mat Yunus Abdul, Fizree, MD Piji Mohd Al-Akmarul, Bahariah, Bohari, Shaharuddin, Noor Azmi, Ho, Chai Ling, Rasid, Omar Abdul, Parveez, Ghulam Kadir Ahmad
• Format: Article
• Published: Elsevier 2024
• Online Access: http://psasir.upm.edu.my/id/eprint/105666/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85177041411&doi=10.1016%2fj.indcrop.2023.117795&partnerID=40&md5=0206d35d04a31400ace6a108638808c0

CRISPR/Cas9 is a powerful genome editing tool applicable to diverse plant species. However, the application of CRISPR/Cas9-ribonucleoproteins (RNPs) in oil palm (Elaeis guineensis) has not yet been reported. In this paper, the specificity, efficiency, and sensitivity of CRISPR/Cas9-RNPs in oil palm...