Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles
Vandachostylis ‘Sri-Siam’ is a fragrant orchid, suitable as a potted plant for the floriculture and perfumery industries due to its sweet citrus-like fragrance. Fragrance from the flowers was captured using SPME and subjected to GC–MS analysis. A transcriptome library was constructed from the tepals...
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Springer Science and Business Media LLC
2022
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my.upm.eprints.1025842024-03-21T09:14:27Z http://psasir.upm.edu.my/id/eprint/102584/ Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles Mohd Hairul, Ab. Rahim Ong-Abdullah, Janna Song, Adelene Ai Lian Foo, Hooi Ling Namasivayam, Parameswari Rahim, Raha Abdul Vandachostylis ‘Sri-Siam’ is a fragrant orchid, suitable as a potted plant for the floriculture and perfumery industries due to its sweet citrus-like fragrance. Fragrance from the flowers was captured using SPME and subjected to GC–MS analysis. A transcriptome library was constructed from the tepals for sequencing using the Illumina MiSeq Desktop Sequencer and for de novo assembly using the CLC Genomic Workbench version 6.0. All de novo assembly sequences were subjected to bioinformatics analyses including BLASTx analysis, functional annotations and mapping of the annotated genes/gene products to the KEGG database using the Blast2go Pro software. GC–MS analysis reveals the fragrance are dominated by 68.73% monoterpenes, 11.26% esters, 4.20% sesquiterpenes and traces of alcohols (1.20%), dissimilar to both its parents. Analysis of the de novo assembly sequences generated 45,585 contigs which identified 14 fragrance-related transcripts. Information on the fragrance constituents and fragrance-related transcripts is deemed important as a reference for current and future work of using this hybrid in the breeding process of commercially valuable fragrant orchids. Springer Science and Business Media LLC 2022-05 Article PeerReviewed Mohd Hairul, Ab. Rahim and Ong-Abdullah, Janna and Song, Adelene Ai Lian and Foo, Hooi Ling and Namasivayam, Parameswari and Rahim, Raha Abdul (2022) Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles. Journal of Plant Biochemistry and Biotechnology, 31 (4). pp. 965-968. ISSN 0971-7811; ESSN: 0974-1275 https://link.springer.com/article/10.1007/s13562-022-00783-4 10.1007/s13562-022-00783-4 |
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Vandachostylis ‘Sri-Siam’ is a fragrant orchid, suitable as a potted plant for the floriculture and perfumery industries due to its sweet citrus-like fragrance. Fragrance from the flowers was captured using SPME and subjected to GC–MS analysis. A transcriptome library was constructed from the tepals for sequencing using the Illumina MiSeq Desktop Sequencer and for de novo assembly using the CLC Genomic Workbench version 6.0. All de novo assembly sequences were subjected to bioinformatics analyses including BLASTx analysis, functional annotations and mapping of the annotated genes/gene products to the KEGG database using the Blast2go Pro software. GC–MS analysis reveals the fragrance are dominated by 68.73% monoterpenes, 11.26% esters, 4.20% sesquiterpenes and traces of alcohols (1.20%), dissimilar to both its parents. Analysis of the de novo assembly sequences generated 45,585 contigs which identified 14 fragrance-related transcripts. Information on the fragrance constituents and fragrance-related transcripts is deemed important as a reference for current and future work of using this hybrid in the breeding process of commercially valuable fragrant orchids. |
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Mohd Hairul, Ab. Rahim Ong-Abdullah, Janna Song, Adelene Ai Lian Foo, Hooi Ling Namasivayam, Parameswari Rahim, Raha Abdul |
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Mohd Hairul, Ab. Rahim Ong-Abdullah, Janna Song, Adelene Ai Lian Foo, Hooi Ling Namasivayam, Parameswari Rahim, Raha Abdul Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles |
author_facet |
Mohd Hairul, Ab. Rahim Ong-Abdullah, Janna Song, Adelene Ai Lian Foo, Hooi Ling Namasivayam, Parameswari Rahim, Raha Abdul |
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Mohd Hairul, Ab. Rahim |
title |
Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles |
title_short |
Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles |
title_full |
Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles |
title_fullStr |
Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles |
title_full_unstemmed |
Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles |
title_sort |
vandachostylis ‘sri-siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles |
publisher |
Springer Science and Business Media LLC |
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2022 |
url |
http://psasir.upm.edu.my/id/eprint/102584/ https://link.springer.com/article/10.1007/s13562-022-00783-4 |
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1794564349819355136 |
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13.188404 |