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Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises

This technical note aims to describe the venipuncture procedure used to collect blood from Southern River terrapins via the subcarapacial venous plexus (SVP) and jugular vein. On uncooperative terrapins, SVP was applied while the jugular vein was reversed. 1.5 ml blood was preserved in 0.5 ml EDTA a...

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Main Authors: Mohd Salleh, Mohd Hairul, Esa, Yuzine
Format: Article
Published: Iranian society of Ichthyology 2022
Online Access:http://psasir.upm.edu.my/id/eprint/102182/
https://ij-aquaticbiology.com/index.php/ijab/article/view/1552
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spelling my.upm.eprints.1021822023-07-10T01:27:08Z http://psasir.upm.edu.my/id/eprint/102182/ Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises Mohd Salleh, Mohd Hairul Esa, Yuzine This technical note aims to describe the venipuncture procedure used to collect blood from Southern River terrapins via the subcarapacial venous plexus (SVP) and jugular vein. On uncooperative terrapins, SVP was applied while the jugular vein was reversed. 1.5 ml blood was preserved in 0.5 ml EDTA and stored at -20°C. ReliaPrepTM Blood genomic DNA Miniprep was used to extract DNA. Thermo ScientificTM NanoDrop 2000c was used to determine the concentrations of extracted DNAs. The greatest concentration of DNA is 136.3 g/L, and the highest purity is 1.90. The treatment is safe, minimally invasive, and effective. Iranian society of Ichthyology 2022-06-14 Article PeerReviewed Mohd Salleh, Mohd Hairul and Esa, Yuzine (2022) Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises. International Journal of Aquatic Biology, 10 (2). 145 - 150. ISSN 2383-0956; ESSN: 2322-5270 https://ij-aquaticbiology.com/index.php/ijab/article/view/1552
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description This technical note aims to describe the venipuncture procedure used to collect blood from Southern River terrapins via the subcarapacial venous plexus (SVP) and jugular vein. On uncooperative terrapins, SVP was applied while the jugular vein was reversed. 1.5 ml blood was preserved in 0.5 ml EDTA and stored at -20°C. ReliaPrepTM Blood genomic DNA Miniprep was used to extract DNA. Thermo ScientificTM NanoDrop 2000c was used to determine the concentrations of extracted DNAs. The greatest concentration of DNA is 136.3 g/L, and the highest purity is 1.90. The treatment is safe, minimally invasive, and effective.
format Article
author Mohd Salleh, Mohd Hairul
Esa, Yuzine
spellingShingle Mohd Salleh, Mohd Hairul
Esa, Yuzine
Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises
author_facet Mohd Salleh, Mohd Hairul
Esa, Yuzine
author_sort Mohd Salleh, Mohd Hairul
title Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises
title_short Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises
title_full Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises
title_fullStr Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises
title_full_unstemmed Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises
title_sort minimally invasive blood collection techniques as a source of gdna for genetic studies on turtles and tortoises
publisher Iranian society of Ichthyology
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/102182/
https://ij-aquaticbiology.com/index.php/ijab/article/view/1552
_version_ 1772813429029994496
score 13.211869

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