Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay
Background: Orthosiphon aristatus has been usually related to be powerful in curing many illnesses inclusive of post-partum remedy, anti-influence, rheumatism and stopping osteoporosis as a result of menopause. Objectives: Thus, this study was designed to differentiate the toxicity effect of non-irr...
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Walailak University
2022
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my.upm.eprints.1013172023-10-06T23:14:48Z http://psasir.upm.edu.my/id/eprint/101317/ Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay Balan, Santhra Segaran Manokaram, Punithaamalar Maya, Sitell Bahari, Hasnah Mohd Uzid, Mahathir Muhammad Danial, Muhammad Danial Zainal Abidin, Azrina Jesus, Arapoc Daryl Rahmadi, Mahardian Background: Orthosiphon aristatus has been usually related to be powerful in curing many illnesses inclusive of post-partum remedy, anti-influence, rheumatism and stopping osteoporosis as a result of menopause. Objectives: Thus, this study was designed to differentiate the toxicity effect of non-irradiated and irradiated Orthosiphon aristatus at different dosage of 3, 6, 9 and 12 kilogray (kGy) on zebrafish embryo. Method: Survival rate, hatching rate, heart beat rate and scoliosis were observed and date were analysed using SPSS 25.00 windows. Result: The lethal dose (LC50) of non-irradiated Orthosiphon aristatus is 381.81 μg/mL compare to irradiated extract at 3 % is (371.27 μg/mL), 6 % (311.03 μg/mL), 9 % (160.72 μg/mL) and 12 % (140.18 μg/mL). Hatchability of zebrafish embryo for Orthosiphon aristatus extract decrease in the higher dosage of irradiated sample compared to non-irradiated sample. No presence of scoliosis was observed in all dosage of irradiated and non-irradiated of Orthosiphon aristatus. The heartbeat of zebrafish embryo treated with irradiated Orthosiphon aristatus specifically 12 % show decrease at 250 μg/mL concentration. Remaining dosage of irradiated and non-irradiated Orthosiphon aristatus show average heartbeat around 120-160 bpm. Conclusion: As conclusion, irradiated and non-irradiated of Orthosiphon aristatus is safe to be consumed due to its pharmaceutical effect but it still exhibited mild toxicity effect on zebrafish embryo. Walailak University 2022 Article PeerReviewed Balan, Santhra Segaran and Manokaram, Punithaamalar and Maya, Sitell and Bahari, Hasnah and Mohd Uzid, Mahathir and Muhammad Danial, Muhammad Danial and Zainal Abidin, Azrina and Jesus, Arapoc Daryl and Rahmadi, Mahardian (2022) Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay. Trends in Sciences, 19 (14). art. no. 4980. pp. 1-7. ISSN 2774-0226 https://tis.wu.ac.th/index.php/tis/article/view/4980 10.48048/tis.2022.4980 |
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Background: Orthosiphon aristatus has been usually related to be powerful in curing many illnesses inclusive of post-partum remedy, anti-influence, rheumatism and stopping osteoporosis as a result of menopause. Objectives: Thus, this study was designed to differentiate the toxicity effect of non-irradiated and irradiated Orthosiphon aristatus at different dosage of 3, 6, 9 and 12 kilogray (kGy) on zebrafish embryo. Method: Survival rate, hatching rate, heart beat rate and scoliosis were observed and date were analysed using SPSS 25.00 windows. Result: The lethal dose (LC50) of non-irradiated Orthosiphon aristatus is 381.81 μg/mL compare to irradiated extract at 3 % is (371.27 μg/mL), 6 % (311.03 μg/mL), 9 % (160.72 μg/mL) and 12 % (140.18 μg/mL). Hatchability of zebrafish embryo for Orthosiphon aristatus extract decrease in the higher dosage of irradiated sample compared to non-irradiated sample. No presence of scoliosis was observed in all dosage of irradiated and non-irradiated of Orthosiphon aristatus. The heartbeat of zebrafish embryo treated with irradiated Orthosiphon aristatus specifically 12 % show decrease at 250 μg/mL concentration. Remaining dosage of irradiated and non-irradiated Orthosiphon aristatus show average heartbeat around 120-160 bpm. Conclusion: As conclusion, irradiated and non-irradiated of Orthosiphon aristatus is safe to be consumed due to its pharmaceutical effect but it still exhibited mild toxicity effect on zebrafish embryo. |
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Article |
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Balan, Santhra Segaran Manokaram, Punithaamalar Maya, Sitell Bahari, Hasnah Mohd Uzid, Mahathir Muhammad Danial, Muhammad Danial Zainal Abidin, Azrina Jesus, Arapoc Daryl Rahmadi, Mahardian |
spellingShingle |
Balan, Santhra Segaran Manokaram, Punithaamalar Maya, Sitell Bahari, Hasnah Mohd Uzid, Mahathir Muhammad Danial, Muhammad Danial Zainal Abidin, Azrina Jesus, Arapoc Daryl Rahmadi, Mahardian Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay |
author_facet |
Balan, Santhra Segaran Manokaram, Punithaamalar Maya, Sitell Bahari, Hasnah Mohd Uzid, Mahathir Muhammad Danial, Muhammad Danial Zainal Abidin, Azrina Jesus, Arapoc Daryl Rahmadi, Mahardian |
author_sort |
Balan, Santhra Segaran |
title |
Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay |
title_short |
Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay |
title_full |
Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay |
title_fullStr |
Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay |
title_full_unstemmed |
Evaluation an aqueous extract of irradiated and non irradiated of Orthosiphon aristatus on zebrafish embryo (Danio rerio) through acute toxicity assay |
title_sort |
evaluation an aqueous extract of irradiated and non irradiated of orthosiphon aristatus on zebrafish embryo (danio rerio) through acute toxicity assay |
publisher |
Walailak University |
publishDate |
2022 |
url |
http://psasir.upm.edu.my/id/eprint/101317/ https://tis.wu.ac.th/index.php/tis/article/view/4980 |
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1781706692417814528 |
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13.160551 |