Designing gRNAs targeting oil palm phytoene desaturase (EgPDS) gene and construction of vectors for oil palm CRISPR/Cas9 study

• Main Authors: Jamaludin, Norfaezah, Bahariah, Bohari, Shaharuddin, Noor Azmi, Chai, Ling Ho, Abdul Rasid, Omar, Ahmad Parveez, Ghulam Kadir, Abdul Masani, Mat Yunus
• Format: Article
• Published: Malaysian Palm Oil Board 2022
• Online Access: http://psasir.upm.edu.my/id/eprint/100905/; http://jopr.mpob.gov.my/designing-grnas-targeting-oil-palm-phytoene-desaturase-iegpdsi-gene-and-construction-of-vectors-for-oil-palm-crisprcas9-study/

The CRISPR/Cas9 system is a precise and versatile genetic tool used to induce site-specific manipulation in the targeted gene. This study elaborates on the strategy to select the gRNAs targeting the oil palm phytoene desaturase (EgPDS) gene, which complements CRISPR-GE and sequence analysis. Sixty-o...