Comparison of real-time PCR, conventional PCR and RT-lamp for the detection of Coconut Cadang-Cadang Viroid variant in oil palm

• Main Authors: Roslan, Nur Diyana, Vadamalai, Ganesan, Idris, Abu Seman, Kong, Lih Ling, Sundram, Shamala
• Format: Article
• Published: Malaysian Palm Oil Board 2023
• Online Access: http://psasir.upm.edu.my/id/eprint/100759/; http://jopr.mpob.gov.my/comparison-of-real-time-pcr-conventional-pcr-and-rt-lamp-for-the-detection-of-icoconut-cadang-cadang-viroidi-variant-in-oil-palm/

Real-time quantitative polymerase chain reaction (real-time PCR) was designed for the detection of oil palm Coconut Cadang-cadang Viroid (CCCVd) 246 nt variant. The primers and probe specifically designed for the real-time PCR were optimised with 300 nM of probe against a primer concentration of 400...