Antagonistic activity of wild growing mushrooms against various fungal rice pathogen

• Main Authors: Mohd-Baseri, Nurkhalida, Kheirel Anuar, Muhammad Salahudin, Shamsuhazli, Nur Alia Syamimi, Zulkifli, Mohd Amirul Faiz, Wasoh, Helmi, Yusof, Mohd Termizi
• Format: Article
• Published: Springer Nature Switzerland 2022
• Online Access: http://psasir.upm.edu.my/id/eprint/100275/; https://link.springer.com/article/10.1007/s10123-022-00275-4

Paddy is an important crop in Malaysia. There are various pathogens able to infect paddy causing a loss in yield’s production. In this study, dual culture method, volatile organic compound (VOC) analysis, and non-volatile compound analysis were used to assess the ability of mushroom to control fungal rice pathogens including Curvularia lunata, Bipolaris panici-miliacei, and Nigrospora sp. Four mushroom isolates were further analysed for their antagonistic activity against rice pathogen. The highest percentage inhibition of radial growth (PIRG) was recorded between 45.55 and 73.68% observed in isolate 42b. The 4 isolates with the highest PIRG based on the dual culture analysis were then tested for their production of VOCs and non-volatile compound. Internal transcribed spacer (ITS) region analysis of the 4 mushroom isolates revealed their identity as Coprinellus disseminates (isolate 12b), Marasmiellus palmivorus (isolate 42b), Trametes maxima (isolate 56e), and Lentinus sajor-caju (isolate 60a). This study showed that mushroom isolates have the potential of antagonistic effect on various fungal rice pathogens tested by the production of secondary metabolites and mycoparasitic interaction.