Purification of lipase from Burkholderia metallica fermentation broth in a column chromatography using polymer impregnated resins

• Main Authors: Ng Z.J., Abbasiliasi S., Yew Joon T., Ng H.S., Phapugrangkul P., Tan J.S.
• Other Authors: 57212007643
• Format: Article
• Published: Taylor and Francis Ltd. 2024
• Subjects: Burkholderia metallica; column chromatography; lipase; PEG grafted porous glass beads; purification; Chromatography; Fermentation; Hydrogen-Ion Concentration; Lipase; Polyethylene Glycols; Polymers; macrogol; polymer; triacylglycerol lipase; chemistry; chromatography; fermentation; pH

In this work, porous glass beads grafted with polyethylene glycol (PEG) were used as an adsorbent to purify lipase from Burkholderia metallica in column chromatography. The purification parameters viz. salt stability, types and concentrations of PEG and salt, pH of the binding solution, and flow rat...