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Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system

Antioxidants; Flotation; Liquids; Molecular biology; Bio-compounds; Bioprocessing; Bubble separation; Chaetoceros calcitrans; Electropermeabilization; Electropermeabilization-assisted; Electroporation; Extraction systems; Fucoxanthin; Liquid biphasic flotation; Extraction; alcohol; copper; fucoxanth...

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Main Authors: Khoo K.S., Ooi C.W., Chew K.W., Chia S.R., Foo S.C., Ng H.S., Show P.L.
Other Authors: 57209198778
Format: Article
Published: Elsevier B.V. 2023
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spelling my.uniten.dspace-269112023-05-29T17:37:43Z Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system Khoo K.S. Ooi C.W. Chew K.W. Chia S.R. Foo S.C. Ng H.S. Show P.L. 57209198778 57216184956 57192980692 57194081866 56779646200 48861933800 47861451300 Antioxidants; Flotation; Liquids; Molecular biology; Bio-compounds; Bioprocessing; Bubble separation; Chaetoceros calcitrans; Electropermeabilization; Electropermeabilization-assisted; Electroporation; Extraction systems; Fucoxanthin; Liquid biphasic flotation; Extraction; alcohol; copper; fucoxanthin; sodium chloride; fucoxanthin; xanthophyll; antioxidant activity; Article; biomass; bioprocess; Chaetoceros; Chaetoceros calcitrans; concentration (parameter); controlled study; diatom; electric potential; electroporation; extraction; flotation; flow rate; liquid biphasic flotation; nonhuman; partition coefficient; diatom; electroporation; microalga; Diatoms; Electroporation; Microalgae; Xanthophylls Electropermeabilization-assisted liquid biphasic flotation (LBF) system is an emerging extraction system facilitated by the adsorptive bubble separation and the electroporation in an aqueous two-phase medium. This integrative extraction system is well suited for the direct recovery of intracellular biocompounds from cells without the needs of discrete steps of mid- or down-stream bioprocessing. The potential of electropermeabilization-assisted LBF system in bioseparation was demonstrated in this study by using the diatom Chaetoceros calcitrans (C. calcitrans) as the source of fucoxanthin, which is a promising antioxidant highly demanded by food and pharmaceutical industries. The extraction performances of LBF and electropermeabilization-assisted LBF system were comprehensively evaluated under the optimal operating conditions. Comparatively, the optimized LBF and electropermeabilization-assisted LBF systems achieved the satisfactory yields of fucoxanthin, i.e., 14.78 mg/g and 16.09 mg/g, respectively. The good recovery of fucoxanthin using electropermeabilization-assisted LBF system could be attributed to the higher release of fucoxanthin from the electrotreated C. calcitrans, allowing a higher partition of fucoxanthin to the top phase of LBF system (236.72 as compared to 152.15 from LBF system). In addition, the fucoxanthin extracted suing both methods exhibit satisfactory antioxidant activities. The application of electropermeabilization-assisted LBF system in the extraction of fucoxanthin is a greener and highly efficient bioprocessing route which can be prospectively extended to other biocompound extraction from microalgal sources. � 2022 Elsevier B.V. Final 2023-05-29T09:37:43Z 2023-05-29T09:37:43Z 2022 Article 10.1016/j.chroma.2022.462915 2-s2.0-85125619442 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125619442&doi=10.1016%2fj.chroma.2022.462915&partnerID=40&md5=81abad1f4bea39a0886ce906deaec91f https://irepository.uniten.edu.my/handle/123456789/26911 1668 462915 Elsevier B.V. Scopus
institution Universiti Tenaga Nasional
building UNITEN Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tenaga Nasional
content_source UNITEN Institutional Repository
url_provider http://dspace.uniten.edu.my/
description Antioxidants; Flotation; Liquids; Molecular biology; Bio-compounds; Bioprocessing; Bubble separation; Chaetoceros calcitrans; Electropermeabilization; Electropermeabilization-assisted; Electroporation; Extraction systems; Fucoxanthin; Liquid biphasic flotation; Extraction; alcohol; copper; fucoxanthin; sodium chloride; fucoxanthin; xanthophyll; antioxidant activity; Article; biomass; bioprocess; Chaetoceros; Chaetoceros calcitrans; concentration (parameter); controlled study; diatom; electric potential; electroporation; extraction; flotation; flow rate; liquid biphasic flotation; nonhuman; partition coefficient; diatom; electroporation; microalga; Diatoms; Electroporation; Microalgae; Xanthophylls
author2 57209198778
author_facet 57209198778
Khoo K.S.
Ooi C.W.
Chew K.W.
Chia S.R.
Foo S.C.
Ng H.S.
Show P.L.
format Article
author Khoo K.S.
Ooi C.W.
Chew K.W.
Chia S.R.
Foo S.C.
Ng H.S.
Show P.L.
spellingShingle Khoo K.S.
Ooi C.W.
Chew K.W.
Chia S.R.
Foo S.C.
Ng H.S.
Show P.L.
Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system
author_sort Khoo K.S.
title Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system
title_short Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system
title_full Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system
title_fullStr Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system
title_full_unstemmed Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system
title_sort extraction of fucoxanthin from chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system
publisher Elsevier B.V.
publishDate 2023
_version_ 1806427678381703168
score 13.214268

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