Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications
Alcohols; Enzyme immobilization; Glucose; Glucose oxidase; Glucose sensors; Palm oil; Radioactive waste vitrification; Spheres; Biosensing applications; Glucose biosensor; Glucose concentration; Glucose oxidases (GOx); Non-surfactant Template; Optimum temperature; Physical adsorption; Silica Sphere;...
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Trans Tech Publications Ltd
2023
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my.uniten.dspace-229622023-05-29T14:13:46Z Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications Saiful Anuar N.F. Misran H. Ahmad Salim M. Othman S.Z. 57189697186 6506899840 57189697454 55548997100 Alcohols; Enzyme immobilization; Glucose; Glucose oxidase; Glucose sensors; Palm oil; Radioactive waste vitrification; Spheres; Biosensing applications; Glucose biosensor; Glucose concentration; Glucose oxidases (GOx); Non-surfactant Template; Optimum temperature; Physical adsorption; Silica Sphere; Silica In this study, silica spheres (SiO2) nanomaterials were introduced as the support materials for glucose sensors. Glucose oxidase (GOx) was immobilized onto SiO2 spheres (SiO2/GOx) using physical adsorption (electrostatic interaction) method. The SiO2 spheres were synthesized in-house using a nonsurfactant template of palm oil derived fatty alcohols (PODFA). Based on FT-IR analyses, SiO2/GOx exhibited N-H absorbance at ca. 2990 cm-1 and O-H absorbance at ca. 3330 cm-1. One new absorbance peak was observed at ca. 2320 cm1 attributed to the bending vibration (?bend)of silane molecules (Si-H) obtained by the interaction of GOx and the surface silanols (Si-OH). UV-Vis analysis results exihibited the presence of a new broad peak for GOx/SiO2 sample at ca. 250 nm to 280 nm suggested to be assigned to H2O2, C=C and benzene ring from GOx. The optimum pH and optimum temperature for SiO2/GOx for GOx activity were ca. 7.0 and ca. 50 �C respectively. The increase of glucose concentration from 2mM to 5mM resulted to the increment of absorbance value for both GOx and SiO2/GOx suggesting succesful immobilization of GOx on SiO2 spheres. These materials were suitable for glucose detection at very small glucose concentrations particularly in salivary glucose detection. � 2016 Trans Tech Publications, Switzerland. Final 2023-05-29T06:13:45Z 2023-05-29T06:13:45Z 2016 Conference Paper 10.4028/www.scientific.net/KEM.694.67 2-s2.0-84974731264 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84974731264&doi=10.4028%2fwww.scientific.net%2fKEM.694.67&partnerID=40&md5=763353a39bdf4245f0837858dce0a252 https://irepository.uniten.edu.my/handle/123456789/22962 694 67 71 Trans Tech Publications Ltd Scopus |
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| description |
Alcohols; Enzyme immobilization; Glucose; Glucose oxidase; Glucose sensors; Palm oil; Radioactive waste vitrification; Spheres; Biosensing applications; Glucose biosensor; Glucose concentration; Glucose oxidases (GOx); Non-surfactant Template; Optimum temperature; Physical adsorption; Silica Sphere; Silica |
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57189697186 |
| author_facet |
57189697186 Saiful Anuar N.F. Misran H. Ahmad Salim M. Othman S.Z. |
| format |
Conference Paper |
| author |
Saiful Anuar N.F. Misran H. Ahmad Salim M. Othman S.Z. |
| spellingShingle |
Saiful Anuar N.F. Misran H. Ahmad Salim M. Othman S.Z. Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications |
| author_sort |
Saiful Anuar N.F. |
| title |
Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications |
| title_short |
Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications |
| title_full |
Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications |
| title_fullStr |
Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications |
| title_full_unstemmed |
Facile method of enzyme immobilization on silica quasi-nanospheres (SiO2) synthesized using PODFA for biosensing applications |
| title_sort |
facile method of enzyme immobilization on silica quasi-nanospheres (sio2) synthesized using podfa for biosensing applications |
| publisher |
Trans Tech Publications Ltd |
| publishDate |
2023 |
| _version_ |
1806427860840218624 |
| score |
13.188404 |