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Optical Characterization of Microalgae Using Optical Spectroscopy and Fluorescence Measurement for Real-Time Monitoring of the Density and Growth Rate of Microalgae

FYP 2 SEM 2 2019/2020

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Main Author: Eric Pradana Putra bin Amin
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Language:English
Published: 2023
Subjects:
Spectroscopy
microalgae
monitoring
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spelling my.uniten.dspace-212572023-05-04T14:37:20Z Optical Characterization of Microalgae Using Optical Spectroscopy and Fluorescence Measurement for Real-Time Monitoring of the Density and Growth Rate of Microalgae Eric Pradana Putra bin Amin Spectroscopy microalgae monitoring FYP 2 SEM 2 2019/2020 When microalgae reach their stationary growth phase, optimum production for biofuel, food supplements and pharmaceutical products can be obtained if they are harvested at the beginning of the stationary phase. However, external factors in an outdoor environment such as different sunlight exposure and water content, the density and growth rate of microalgae can vary significantly, and this may pose a challenge to industries in creating a plan on growing and harvesting microalgae. Therefore, there is a need to design a low-cost real-time monitoring system that can provide information on the density and growth of microalgae. This thesis reports a project that investigates the relationship between optical characteristics of tropical microalgae and their growth with the purpose that the results from the investigation can become a reference for the future development of an optical detection system for real-time monitoring of tropical microalgae. Two different species of microalgae at different growth phases were characterized using the spectrometer from 330 to 1020 nm and their refractive indices were also measured using a refractometer. The results showed that the Scenedesmus sp. absorbs light at 427.2, 884.2 and 953.5 nm and the Monoraphidium Griffithii sp absorbs light at 435.9, 685.1, 883.1 and 954.3 nm. Analysis and correlation of the optical spectrum with the growth phases were carried out to determine the optimum wavelengths or waveband that is suitable for the monitoring of microalgae growth. It was found that the 954 nm wavelength is the most suitable wavelength for monitoring the microalgae growth as it shows clear trend from the early growth phase and shows good correlation with the microalgae cell density. Refractive indices for both species showed decreasing trend throughout the project, probably due to physical property changes in the microalgae cells. The results from the refractive index measurements were discussed to determine if the microalgae refractive index has any relationship with their growth phase. 3D scatter plots of absorbance against cell density against refractive index were generated to create mathematical models that can be used to describe the relationship between the three variables. The outcome of the project will lead to the design of real-time microalgae monitoring system. 2023-05-03T16:22:28Z 2023-05-03T16:22:28Z 2020-02 https://irepository.uniten.edu.my/handle/123456789/21257 en application/pdf
institution Universiti Tenaga Nasional
building UNITEN Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tenaga Nasional
content_source UNITEN Institutional Repository
url_provider http://dspace.uniten.edu.my/
language English
topic Spectroscopy
microalgae
monitoring
spellingShingle Spectroscopy
microalgae
monitoring
Eric Pradana Putra bin Amin
Optical Characterization of Microalgae Using Optical Spectroscopy and Fluorescence Measurement for Real-Time Monitoring of the Density and Growth Rate of Microalgae
description FYP 2 SEM 2 2019/2020
format
author Eric Pradana Putra bin Amin
author_facet Eric Pradana Putra bin Amin
author_sort Eric Pradana Putra bin Amin
title Optical Characterization of Microalgae Using Optical Spectroscopy and Fluorescence Measurement for Real-Time Monitoring of the Density and Growth Rate of Microalgae
title_short Optical Characterization of Microalgae Using Optical Spectroscopy and Fluorescence Measurement for Real-Time Monitoring of the Density and Growth Rate of Microalgae
title_full Optical Characterization of Microalgae Using Optical Spectroscopy and Fluorescence Measurement for Real-Time Monitoring of the Density and Growth Rate of Microalgae
title_fullStr Optical Characterization of Microalgae Using Optical Spectroscopy and Fluorescence Measurement for Real-Time Monitoring of the Density and Growth Rate of Microalgae
title_full_unstemmed Optical Characterization of Microalgae Using Optical Spectroscopy and Fluorescence Measurement for Real-Time Monitoring of the Density and Growth Rate of Microalgae
title_sort optical characterization of microalgae using optical spectroscopy and fluorescence measurement for real-time monitoring of the density and growth rate of microalgae
publishDate 2023
_version_ 1806424273426841600
score 13.214268

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