Detection of Listeria spp. and Listeria monocytogenes from small animals, soil and water samples in sarawak
Listeria monocytogenes (L. monocytogenes) is a pathogenic Gram positive bacterium which causes a type of disease called listeriosis in humans. The common sources of infection by L. monocytogenes can be from anima'ls, contaminated water as well as undercooked food. The main objective of this r...
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Main Author: | |
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Format: | Final Year Project Report |
Language: | English |
Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2013
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/8736/3/Hu%20Li%20Tze.pdf http://ir.unimas.my/id/eprint/8736/ |
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Summary: | Listeria monocytogenes (L. monocytogenes) is a pathogenic Gram positive bacterium which causes a type of
disease called listeriosis in humans. The common sources of infection by L. monocytogenes can be from anima'ls,
contaminated water as well as undercooked food. The main objective of this research was to determine the
presence of L. monocytogenes in small mammals, soil and water samples from the selected sites in Sarawak
namely Sebangkoi Recreational Park (Sarikei), Matang Kubah National Park (Kuching) and Bako National Park
(Kuching). The samples collected were enriched in Tryptone Soy Broth (TSB) while P ALCAM agar was used
for the selective isolation of Listeria spp. Upon growth on PALCAM agar, Listeria spp. produced colonies
which were greyish green with black halo. The presence of L. monocytogenes was further confirmed with
Polymerase chain reaction (PCR) to identify the virulence-associated gene in this pathogen. The hlyA virulence
gene in L. monoc;ytogenes codes for a specific enzyme called listeriolysin 0 (LLO) which lyses macrophages by
the secretion of pore-forming toxin. Overall, 38.6% of the isolates obtained from the samples at the three
selected sites in Sarawak contained Listeria spp. However, L. monocytogenes was not detected from any of the
samples examined. Therefore, the wildlife hosts from the three selected sites are free from the pathogen L.
monocytogenes. |
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