Screening of Grb10 interacting GYF domain protein 1b (GIGYF 1b) gene in zebrafish (danio rerio) development

• Main Author: Pang, Shek Li
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/8223/1/Screening%20of%20Grb10%20Interacting%20GYF%20Domain%20Protein%201b%20%28GIGYF%201b%29%20Gene%20in%20Zebrafish%20%28Danio%20rerio%29%20Development%2824pgs%29.pdf; http://ir.unimas.my/id/eprint/8223/8/Pang%20Shek%20Li.pdf; http://ir.unimas.my/id/eprint/8223/

GrblO Interacting GYF domain (GIGYF 1) and Grb 10 interacting GYF domain 2 belong to GIGYF family genes. These two genes interact with Grb I 0, an adapter protein activate Insulin-like Growth Factor-I (lGF-I) through two-hybrid screening. Function of these two genes is regulating tyrosine kinase receptor signaling including IGF-I and insulin signaling. Recently, GIGYF 2 has been nominated as candidate gene involved in Parkinson's disease (PO) as it fallen within PARK II locus, thus, GIGYF 1 was believed it is related in PO since GIGYF 1 is homologous with GIGYF 2. However, functions of GIGYF 1 are remain largely unknown, thus this study might give a hint of function of GIGYF 1. Zebrafish is the organism that suitable for human disease study because zebrafish will often have two GIGYF 1 genes that are GIGYF la and GIGYF I b which are both orthologs of a single mammalian gene. The GIGYF I has an extra tetraploidization with the occurrence of genome duplication. Duplication-Degenerative-Complementation event enable further study of GIGYF I gene, including its functional role in PD. GIGYF I b gene was cloned into pGEM®-T Easy vector and the plasmid with the inserted gene was extracted and stored and ready for probe synthesis for in situ hybridization.