DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta
Studies were conducted to study the optimization of the extraction of DNA and mitochondrial COl gene in two species of the genus Bella; Bella splendens and Betta unimaculata. A total of 15 individuals from Betta fish have been taken from Temerloh, UNIMAS campus, and several locations in Kuching as...
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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Online Access: | http://ir.unimas.my/id/eprint/8135/3/Aisyah%20Binti%20Shamsuddin%20ft.pdf http://ir.unimas.my/id/eprint/8135/ |
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my.unimas.ir.81352024-06-13T04:51:49Z http://ir.unimas.my/id/eprint/8135/ DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta Aisyah, Binti Shamsuddin Q Science (General) Studies were conducted to study the optimization of the extraction of DNA and mitochondrial COl gene in two species of the genus Bella; Bella splendens and Betta unimaculata. A total of 15 individuals from Betta fish have been taken from Temerloh, UNIMAS campus, and several locations in Kuching as well as from local aquarium shops and wet markets. From optimization, eight individuals from 15 samples of Bella fish has been successfuny showed a single band. Various measures have been changed in the form of PCR temperature and time for each steps of PCR to identify the most effective parameters for the PCR product. Initial denaturation of the PCR parameters: 94°C for 3 minutes; annealing: 48.3°C for 1 minute; extension: 72 o C for 1.30 minutes; extension ends: 72 ° C for 5 minutes. 100bp DNA ladder was used to estimate the size of about 520 bp. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/8135/3/Aisyah%20Binti%20Shamsuddin%20ft.pdf Aisyah, Binti Shamsuddin (2012) DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta. [Final Year Project Report] (Unpublished) |
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Q Science (General) Aisyah, Binti Shamsuddin DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta |
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Studies were conducted to study the optimization of the extraction of DNA and mitochondrial COl gene in
two species of the genus Bella; Bella splendens and Betta unimaculata. A total of 15 individuals from Betta
fish have been taken from Temerloh, UNIMAS campus, and several locations in Kuching as well as from
local aquarium shops and wet markets. From optimization, eight individuals from 15 samples of Bella fish
has been successfuny showed a single band. Various measures have been changed in the form of PCR
temperature and time for each steps of PCR to identify the most effective parameters for the PCR product.
Initial denaturation of the PCR parameters: 94°C for 3 minutes; annealing: 48.3°C for 1 minute; extension: 72
o C for 1.30 minutes; extension ends: 72 ° C for 5 minutes. 100bp DNA ladder was used to estimate the size
of about 520 bp. |
format |
Final Year Project Report |
author |
Aisyah, Binti Shamsuddin |
author_facet |
Aisyah, Binti Shamsuddin |
author_sort |
Aisyah, Binti Shamsuddin |
title |
DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta |
title_short |
DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta |
title_full |
DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta |
title_fullStr |
DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta |
title_full_unstemmed |
DNA extraction and PCR optimization of mitochondrial coi gene in the genus betta |
title_sort |
dna extraction and pcr optimization of mitochondrial coi gene in the genus betta |
publisher |
Universiti Malaysia Sarawak, (UNIMAS) |
publishDate |
2012 |
url |
http://ir.unimas.my/id/eprint/8135/3/Aisyah%20Binti%20Shamsuddin%20ft.pdf http://ir.unimas.my/id/eprint/8135/ |
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