Hunting for Second Copy of Proline-Rich EVHI Ligand (PREL) Family Genes in Danio Rerio Genome

• Main Author: Tay, Ai Peng
• Format: E-LPTA
• Language: English; English
• Published: Universiti Malaysia Sarawak, UNIMAS 2010
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/7858/1/Hunting%20for%20Second%20Copy%20of%20Proline-Rich%20EVHI%20Ligand%20%28PREL%29%20Family%20Genes%20in%20Danio%20rerio%20Genome%2024%20pgs.pdf; http://ir.unimas.my/id/eprint/7858/2/Hunting%20for%20Second%20Copy%20of%20Proline-Rich%20EVHI%20Ligand%20%28PREL%29%20Family%20Genes%20in%20Danio%20rerio%20Genome.pdf; http://ir.unimas.my/id/eprint/7858/

Understanding the mechanism of cells migration and proliferation within tissue becomes bottleneck in studying the cell motility and action dynamics. Remodeling of the actin cytoskeleton provides the driving force for cell migration. Profilin and Ena/VASP proteins family were found to be important regulators of cell motility and actin polymerization. Previous studies found that Proline-rich EVH1 Ligand 1(PREL1) as the link between Ras signaling and action cytoskeleton via Ena.VASP proteins while Proline-rich EVH1 Ligand 2 (PREL2) as the ligand for Ena/VASP proteins and profilin which interacts with Rap1-GTP to mediates the Rap1-Induced adhesion. However, the exact mechanism on how profilin and Ena/VASP proteins regulate those processes and details of the molecular interaction involved are remains largely unclear. Hence, in this study, the characteristics and functions of the PREL genes in Danio rerio genome were studied though bioinformatics analysis approach. Second copy of both PREL1 and PREL2 genes were targeted in Danio rerio genome. This study was conducted by performing multiple sequence alignment, phylogeny analysis and three dimensional (3D) structural modeling to confirm the identity of selected genes. CLUSTAL X was used to perform multiple sequence alignment while PAUP* version 4.0b10 and Mr Bayers software were selected for phylogeny analysis. I-TASSER server was employed to construct the 3D models of selected PREL1 and PRE2 sequences. We found that it was two copies of PREL2 genes in Danio rerio genome located in linkage 9 and linkage 1 respectively. However, the present second copy of PREL1 gene still under investigated.