Heterologous expression of cellulase gene from bacillus Amyloliquefacians UMAS 1002 in Escherichia Coli BLl21 (DE3)

• Main Author: Komathi, Balasupramaniam
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, UNIMAS 2010
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/7853/1/Komathi%20Balasupramaniam%20ft.pdf; http://ir.unimas.my/id/eprint/7853/

Cloning and expression of cellulase gene from B. amyloliquefaciens in E. coli BL21 (DE3) was attempted to optimize the activity of recombinant cellulase. PCR amplification of cellulase gene from Top10 cell was not successful although different approaches were carried out. Alternative method was take...