Characterization of Celluloltic fungi in peat soil for potential application and biodegradation of rice husk

• Main Author: Mazurina, Mashoory
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, UNIMAS 2008
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/7636/3/Characterization%20of%20Cellulolytic%20Fungi%20in%20Peat%20Soil%20for%20Potential%20Application%20and%20Biodegradation%20of%20Rice%20Husk.pdf; http://ir.unimas.my/id/eprint/7636/

Cellulases are a group of hydrolytic enzymes that capable of degrading cellulose to the smaller glucose units. Nine isolates were isolated from samples taken from peat soil by growing on Potato Dextrose Agar (PDA). Cellulolytic activities were measured qualitatively and quantitatively by using 0.1% Congo Red and 3,5-dinitrosalicylic acid method, respectively. All isolates were morphologically characterized and the highest cellulase-producing fungus, F9 was selected for species identification by molecular technique. F9 has been identified as Penicillium citrinum was used to proceed the solid state fermentation. The highest cellulolytic activities were shown on day five with; FPase, 1.474 IU/ml; CMCase, 2.663 IU/ml; and Avicelase, 0.449 IU/ml.