Molecular cloning and characterization of partial cDNA encoding for flavanone 3-hydroxylase from kelampayan (neolamarckia cadamba)
Neolamarckia cadamba or Kelampayan is a type of deciduous tree species classified under the Rubiaceae family. The fast fast-growing N. cadamba emerges as a new alternative to meet high global demand of timber wood. Flavanone 3-hydroxylase is one of the key enzymes involved in the flavonoid synthe...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7550/1/Low%20Hui%20Chia%20ft.pdf http://ir.unimas.my/id/eprint/7550/ |
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| Summary: | Neolamarckia cadamba or Kelampayan is a type of deciduous tree species classified
under the Rubiaceae family. The fast fast-growing N. cadamba emerges as a new
alternative to meet high global demand of timber wood. Flavanone 3-hydroxylase is one
of the key enzymes involved in the flavonoid synthesis pathway. Thus, this study aims to
amplify the flavanone 3-hydroxylase partial cDNA in order characterize it through
sequence analysis. High quality total RNA was extracted from the young leaves of N.
cadamba and converted into cDNA. Amplification of cDNA encoding flavanone 3-
hydroxylase was performed through PCR. The BLASTn result of purified PCR product
revealed high similarity with the flavanone 3-hydroxylase (F3H) mRNA of other plant
species, comfirming the identity of the nucleotide sequence as the partial cDNA of
flavanone 3-hydroxylase. |
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