Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line
Ribosomal proteins are essential in protein biosynthesis process and they also have extraribosomal functions. These proteins have been found to be closely associated with various inherited diseases and cancers. Previous studies revealed that the ribosomal protein large subunit (RPL) gene expressed...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7487/8/Comparative%20analysis%20of%20expression%20patterns%20among%20the%20ribosomal%20protein%20genes%20of%20RPL27%20%28OCR%29.pdf http://ir.unimas.my/id/eprint/7487/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Ribosomal proteins are essential in protein biosynthesis process and they also have extraribosomal functions.
These proteins have been found to be closely associated with various inherited diseases and cancers. Previous
studies revealed that the ribosomal protein large subunit (RPL) gene expressed differently among cell line
derived from human nasopharyngeal carcinoma (NPC). However, RPL27, RPL37a, and RPL41 are found to
be significantly downregulated in all cell lines derived from NPC tumours when compared to normal control.
The main objective of this study is to evaluate quantitatively the expressed transcript level among the RP genes of RPL27, RPL37a, and RPL41 in the human cell line system. The method of Reverse Transcription
Polymerase Chain Reaction (RT-PCR) coupled with band intensity analysis will be used to meet this
objective. Result from this finding show that there are no significant different in expressions of RPL27,
RPL37a, and RPL41 in NPC tumours and normal control. Besides that, RPL27 and RPL37a show significant
correlation when increase in sample size. |
|---|