Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river
Sarawak River plays a major role to human especially in the social and economic activities of Sarawak State. Local fishermen are depending very much on the river to catch raw foods, such as fish, for human consumption. The river also provides water for agriculture activities and acts as place for...
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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my.unimas.ir.73712023-06-15T08:38:41Z http://ir.unimas.my/id/eprint/7371/ Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river Ili Liyana, Binti Khlir QR Microbiology SH Aquaculture. Fisheries. Angling Sarawak River plays a major role to human especially in the social and economic activities of Sarawak State. Local fishermen are depending very much on the river to catch raw foods, such as fish, for human consumption. The river also provides water for agriculture activities and acts as place for recreational activities. Coliform bacteria, such as E. coli, have been used as indicator for the microbiological quality of the water and also the fish. In this study, the presence of coliform bacteria in the water and fish samples from Sarawak River were determined and the genetic fingerprint of the bacteria isolates have been used to analyze the genetic diversity of the bacteria in the water and the fish. The samples were collected from three different locations along the Sarawak River; Pending, barrage area, and Waterfront. The coliform bacteria isolated were analyzed and confirmed by biochemical test. Twenty-seven isolates of coliform bacteria were identified. The genetic fingerprint has been carried out by RAPD-PCR analysis. Hence, the study shows that the river is contaminated with coliform bacteria, and the bacteria in the water sample have been found in the fish as well. In term of DNA profile, coliform bacteria in the fish are related to the coliform bacteria in the water. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7371/7/DISTRIBUTION%20..%28fulltext%29.pdf Ili Liyana, Binti Khlir (2012) Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river. [Final Year Project Report] (Unpublished) |
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QR Microbiology SH Aquaculture. Fisheries. Angling Ili Liyana, Binti Khlir Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river |
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Sarawak River plays a major role to human especially in the social and economic activities of Sarawak State.
Local fishermen are depending very much on the river to catch raw foods, such as fish, for human
consumption. The river also provides water for agriculture activities and acts as place for recreational
activities. Coliform bacteria, such as E. coli, have been used as indicator for the microbiological quality of
the water and also the fish. In this study, the presence of coliform bacteria in the water and fish samples from
Sarawak River were determined and the genetic fingerprint of the bacteria isolates have been used to analyze
the genetic diversity of the bacteria in the water and the fish. The samples were collected from three different
locations along the Sarawak River; Pending, barrage area, and Waterfront. The coliform bacteria isolated
were analyzed and confirmed by biochemical test. Twenty-seven isolates of coliform bacteria were
identified. The genetic fingerprint has been carried out by RAPD-PCR analysis. Hence, the study shows that
the river is contaminated with coliform bacteria, and the bacteria in the water sample have been found in the
fish as well. In term of DNA profile, coliform bacteria in the fish are related to the coliform bacteria in the
water. |
format |
Final Year Project Report |
author |
Ili Liyana, Binti Khlir |
author_facet |
Ili Liyana, Binti Khlir |
author_sort |
Ili Liyana, Binti Khlir |
title |
Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river |
title_short |
Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river |
title_full |
Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river |
title_fullStr |
Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river |
title_full_unstemmed |
Distribution and genetic linkage of coliform bacteria from fish and water from Sarawak river |
title_sort |
distribution and genetic linkage of coliform bacteria from fish and water from sarawak river |
publisher |
Universiti Malaysia Sarawak, (UNIMAS) |
publishDate |
2012 |
url |
http://ir.unimas.my/id/eprint/7371/7/DISTRIBUTION%20..%28fulltext%29.pdf http://ir.unimas.my/id/eprint/7371/ |
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1769847604298907648 |
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13.159267 |