Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups

• Main Authors: Wu, Y., Tan, E. L., Yeo, A., Chan, K.P., Nishimura, H., Cardosa, M.J, Poh, C.L., Quak, S.H., Chow, Vincent T.
• Format: E-Article
• Language: English
• Published: American Society for Microbiology 2011
• Subjects: Q Science (General); QR355 Virology; R Medicine (General)
• Online Access: http://ir.unimas.my/id/eprint/6939/1/Novel%20Multiplex%20Oligonucleotide-Conjugated%20Bead%20Suspension%20Array%20for%20Rapid%20Identification%20of%20Enterovirus%2071%20Subgenogroups.pdf; http://ir.unimas.my/id/eprint/6939/; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020481/

A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and correctly subgenogrouped.