High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting
The Malayan tiger (Panthera tigris jacksoni) is a critically endangered species native to the Malaysian Peninsula. To imitate wild conditions where tigers do not hunt every day, numerous wildlife sanctuaries do not feed their tigers daily. However, the effects of fasting on the gut microbiota of cap...
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my.unimas.ir.427382023-10-03T00:20:01Z http://ir.unimas.my/id/eprint/42738/ High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting Mohamad, Khairulmunir Millawati, Gani Kayal Vizi, Karuppannan Abd Rahman, Mohd Ridwan Badrul Munir, Md Zain QL Zoology The Malayan tiger (Panthera tigris jacksoni) is a critically endangered species native to the Malaysian Peninsula. To imitate wild conditions where tigers do not hunt every day, numerous wildlife sanctuaries do not feed their tigers daily. However, the effects of fasting on the gut microbiota of captive Malayan tigers remains unknown. This study aimed to characterise the gut microbiota of captive Malayan tigers by comparing their microbial communities during fasting versus normal feeding conditions. This study was conducted at the Melaka Zoo, Malaysian Peninsula and involved Malayan tigers fasted every Monday. In total, ten faecal samples of Malayan tiger, two of Bengal tiger (outgroup) and four of lion (outgroup) were collected and analysed for metabarcoding targeting the 16S rRNA V3–V4 region. In total, we determined 14 phyla, 87 families, 167 genera and 53 species of gut microbiome across Malayan tiger samples. The potentially harmful bacterial genera found in this study included Fusobacterium, Bacteroides, Clostridium sensu stricto 1,Solobacterium, Echerichia shigella, Ignatzschineria and Negativibacillus. The microbiome in the fasting phase had a higher composition and was more diverse than in the feeding phase. The present findings indicate a balanced ratio in the dominant phyla, reflecting a resetting of the imbalanced gut microbiota due to fasting. These findings can help authorities in how to best maintain and improve the husbandry and health of Malayan tigers in captivity and be used for monitoring in ex-situ veterinary care unit. Pensoft Publishers 2023-09-05 Article PeerReviewed text en http://ir.unimas.my/id/eprint/42738/3/High.pdf Mohamad, Khairulmunir and Millawati, Gani and Kayal Vizi, Karuppannan and Abd Rahman, Mohd Ridwan and Badrul Munir, Md Zain (2023) High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting. Biodiversity Data Journal, 11 (e1047). pp. 1-22. ISSN 1314-2828 https://bdj.pensoft.net/browse_journal_articles_by_author?user_id=141727 doi: 10.3897/BDJ.11.e104757 |
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QL Zoology Mohamad, Khairulmunir Millawati, Gani Kayal Vizi, Karuppannan Abd Rahman, Mohd Ridwan Badrul Munir, Md Zain High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting |
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The Malayan tiger (Panthera tigris jacksoni) is a critically endangered species native to the Malaysian Peninsula. To imitate wild conditions where tigers do not hunt every day, numerous wildlife sanctuaries do not feed their tigers daily. However, the effects of fasting on the gut microbiota of captive Malayan tigers remains unknown. This study aimed to characterise the gut microbiota of captive Malayan tigers by comparing their microbial communities during fasting versus normal feeding conditions. This study was conducted at the Melaka Zoo, Malaysian Peninsula and involved Malayan tigers fasted every Monday. In total, ten faecal samples of Malayan tiger, two of Bengal tiger (outgroup) and four of lion (outgroup) were collected and analysed for metabarcoding targeting the 16S rRNA V3–V4 region. In total, we determined 14 phyla, 87 families, 167 genera and 53 species of gut microbiome across Malayan tiger samples. The potentially harmful bacterial genera found in this study included Fusobacterium, Bacteroides, Clostridium sensu stricto 1,Solobacterium, Echerichia shigella, Ignatzschineria and Negativibacillus. The microbiome in the fasting phase had a higher composition and was more diverse than in the feeding phase. The present findings indicate a balanced ratio in the dominant phyla, reflecting a resetting of the imbalanced gut microbiota due to fasting. These findings can help authorities in how to best maintain and improve the husbandry and health of Malayan tigers in captivity and be used for monitoring in ex-situ veterinary care unit. |
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Article |
author |
Mohamad, Khairulmunir Millawati, Gani Kayal Vizi, Karuppannan Abd Rahman, Mohd Ridwan Badrul Munir, Md Zain |
author_facet |
Mohamad, Khairulmunir Millawati, Gani Kayal Vizi, Karuppannan Abd Rahman, Mohd Ridwan Badrul Munir, Md Zain |
author_sort |
Mohamad, Khairulmunir |
title |
High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting |
title_short |
High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting |
title_full |
High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting |
title_fullStr |
High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting |
title_full_unstemmed |
High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Panthera tigris jacksoni) during fasting |
title_sort |
high-throughput dna metabarcoding for determining the gut microbiome of captive critically endangered malayan tiger (panthera tigris jacksoni) during fasting |
publisher |
Pensoft Publishers |
publishDate |
2023 |
url |
http://ir.unimas.my/id/eprint/42738/3/High.pdf http://ir.unimas.my/id/eprint/42738/ https://bdj.pensoft.net/browse_journal_articles_by_author?user_id=141727 |
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1779150691355328512 |
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13.19449 |