Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis

• Main Authors: Hany, Sady, Hesham M., Al-Mekhlafi, Romano, Ngui, Wahib M., Atroosh, Ahmed K., Al-Delaimy, Nabil A., Nasr, Salwani, Dawaki, Awatif M., Abdulsalam, Init, Ithoi, Yvonne Ai, Lian Lim, Kek, Heng Chua, Johari, Surin
• Format: Article
• Language: English
• Published: MDPI 2015
• Subjects: QR Microbiology; RZ Other systems of medicine
• Online Access: http://ir.unimas.my/id/eprint/42185/1/Detection%20of.pdf; http://ir.unimas.my/id/eprint/42185/; https://www.mdpi.com/1422-0067/16/7/16085; https://doi.org/10.3390/ijms160716085

The present study describes a real-time PCR approach with high resolution melting-curve (HRM) assay developed for the detection and differentiation of Schistosoma mansoni and S. haematobium in fecal and urine samples collected from rural Yemen. The samples were screened by microscopy and PCR for the...