Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5...
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Online Access: | http://ir.unimas.my/id/eprint/41190/1/Random%20amplified%20polymorphic%20DNA-PCR%20and%20ERIC%20PCR%20analysis.pdf http://ir.unimas.my/id/eprint/41190/ |
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my.unimas.ir.411902023-01-26T04:51:29Z http://ir.unimas.my/id/eprint/41190/ Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia Zulkifli, Y Alitheen, N.B Son, R Raha, A.R Samuel, Lihan Yeap, S.K Nishibuchi, Mitsuaki QR Microbiology In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus. 2009 Article PeerReviewed text en http://ir.unimas.my/id/eprint/41190/1/Random%20amplified%20polymorphic%20DNA-PCR%20and%20ERIC%20PCR%20analysis.pdf Zulkifli, Y and Alitheen, N.B and Son, R and Raha, A.R and Samuel, Lihan and Yeap, S.K and Nishibuchi, Mitsuaki (2009) Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. International Food Research Journal, 16 (3). pp. 141-150. |
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QR Microbiology Zulkifli, Y Alitheen, N.B Son, R Raha, A.R Samuel, Lihan Yeap, S.K Nishibuchi, Mitsuaki Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
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In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into
4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus. |
format |
Article |
author |
Zulkifli, Y Alitheen, N.B Son, R Raha, A.R Samuel, Lihan Yeap, S.K Nishibuchi, Mitsuaki |
author_facet |
Zulkifli, Y Alitheen, N.B Son, R Raha, A.R Samuel, Lihan Yeap, S.K Nishibuchi, Mitsuaki |
author_sort |
Zulkifli, Y |
title |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_short |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_full |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_fullStr |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_full_unstemmed |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_sort |
random amplified polymorphic dna-pcr and eric pcr analysis on
vibrio parahaemolyticus isolated from cockles in padang, indonesia |
publishDate |
2009 |
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http://ir.unimas.my/id/eprint/41190/1/Random%20amplified%20polymorphic%20DNA-PCR%20and%20ERIC%20PCR%20analysis.pdf http://ir.unimas.my/id/eprint/41190/ |
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1756063478535159808 |
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13.18916 |