Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia

• Main Authors: Zulkifli, Y, Alitheen, N.B, Son, R, Raha, A.R, Samuel, Lihan, Yeap, S.K, Nishibuchi, Mitsuaki
• Format: Article
• Language: English
• Published: 2009
• Subjects: QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/41190/1/Random%20amplified%20polymorphic%20DNA-PCR%20and%20ERIC%20PCR%20analysis.pdf; http://ir.unimas.my/id/eprint/41190/

In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus.