Osteoblast Demineralization Induced by Oxidized High-Density Lipoprotein via the Inflammatory Pathway Is Suppressed by Adiponectin
Low mineralization activity by human osteoblast cells (HOBs) indicates abnormal bone remodeling that potentially leads to osteoporosis. Oxidation, the most prominent form of high-density lipoprotein (HDL) modification, is suggested to affect bone mineralization through the inflammatory pathway. Adi...
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my.unimas.ir.405592022-11-29T03:11:25Z http://ir.unimas.my/id/eprint/40559/ Osteoblast Demineralization Induced by Oxidized High-Density Lipoprotein via the Inflammatory Pathway Is Suppressed by Adiponectin Noor Hanisa, Harun Gabriele Ruth Anisah, Froemming Aletza, Mohd Ismail Hapizah, Nawawi Siti Shuhada, Mokhtar Suhaila, Abd Muid QD Chemistry R Medicine (General) Low mineralization activity by human osteoblast cells (HOBs) indicates abnormal bone remodeling that potentially leads to osteoporosis. Oxidation, the most prominent form of high-density lipoprotein (HDL) modification, is suggested to affect bone mineralization through the inflammatory pathway. Adiponectin, which possesses anti-inflammatory activity, is postulated to have the ability to suppress the detrimental effects of oxidized HDL (oxHDL). This study aimed to investigate the effects of HDL before and after oxidation on markers of mineralization and inflammation. The protective effects of adiponectin on demineralization and inflammation induced by oxHDL were also investigated. OxHDL at 100 µg/mL protein had the highest inhibitory effect on mineralization, followed by lower calcium incorporation. OxHDL also had significantly lower expression of a mineralization marker (COL1A2) and higher expression of inflammatory markers (IL-6, TNF-α, and RELA proto-oncogene, NF-κβ (p65)) compared to the unstimulated control group. These findings suggest that oxHDL reduces the mineralization activity of HOBs by increasing the expression of inflammatory markers. Interestingly, co-incubation of adiponectin and oxHDL in HOBs resulted in higher expression of mineralization markers (ALPL, COL1A2, BGLAP, and RUNX2) and significantly reduced all targeted inflammatory markers compared to the oxHDL groups. On the contrary, HDL increased the expression of mineralization markers (COL1A2 and STAT-3) and exhibited lower expression of inflammatory cytokines (IL-6 and TNF-α), proving the protective effect of HDL beyond the reverse cholesterol transport activity. MDPI 2022 Article PeerReviewed text en http://ir.unimas.my/id/eprint/40559/1/Osteoblast%20Demineralization%20-%20Copy.pdf Noor Hanisa, Harun and Gabriele Ruth Anisah, Froemming and Aletza, Mohd Ismail and Hapizah, Nawawi and Siti Shuhada, Mokhtar and Suhaila, Abd Muid (2022) Osteoblast Demineralization Induced by Oxidized High-Density Lipoprotein via the Inflammatory Pathway Is Suppressed by Adiponectin. International Journal of Molecular Sciences, 23 (23). pp. 1-16. ISSN 1422-0067 https://www.mdpi.com/1422-0067/23/23/14616/htm https://doi.org/10.3390/ijms232314616 |
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QD Chemistry R Medicine (General) Noor Hanisa, Harun Gabriele Ruth Anisah, Froemming Aletza, Mohd Ismail Hapizah, Nawawi Siti Shuhada, Mokhtar Suhaila, Abd Muid Osteoblast Demineralization Induced by Oxidized High-Density Lipoprotein via the Inflammatory Pathway Is Suppressed by Adiponectin |
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Low mineralization activity by human osteoblast cells (HOBs) indicates abnormal bone remodeling that potentially leads to osteoporosis. Oxidation, the most prominent form of high-density lipoprotein (HDL) modification, is suggested to affect bone mineralization through the inflammatory
pathway. Adiponectin, which possesses anti-inflammatory activity, is postulated to have the ability to suppress the detrimental effects of oxidized HDL (oxHDL). This study aimed to investigate the effects of HDL before and after oxidation on markers of mineralization and inflammation. The
protective effects of adiponectin on demineralization and inflammation induced by oxHDL were also investigated. OxHDL at 100 µg/mL protein had the highest inhibitory effect on mineralization, followed by lower calcium incorporation. OxHDL also had significantly lower expression of a
mineralization marker (COL1A2) and higher expression of inflammatory markers (IL-6, TNF-α, and RELA proto-oncogene, NF-κβ (p65)) compared to the unstimulated control group. These findings suggest that oxHDL reduces the mineralization activity of HOBs by increasing the expression of
inflammatory markers. Interestingly, co-incubation of adiponectin and oxHDL in HOBs resulted in higher expression of mineralization markers (ALPL, COL1A2, BGLAP, and RUNX2) and significantly reduced all targeted inflammatory markers compared to the oxHDL groups. On the contrary, HDL
increased the expression of mineralization markers (COL1A2 and STAT-3) and exhibited lower expression of inflammatory cytokines (IL-6 and TNF-α), proving the protective effect of HDL beyond the reverse cholesterol transport activity. |
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Article |
author |
Noor Hanisa, Harun Gabriele Ruth Anisah, Froemming Aletza, Mohd Ismail Hapizah, Nawawi Siti Shuhada, Mokhtar Suhaila, Abd Muid |
author_facet |
Noor Hanisa, Harun Gabriele Ruth Anisah, Froemming Aletza, Mohd Ismail Hapizah, Nawawi Siti Shuhada, Mokhtar Suhaila, Abd Muid |
author_sort |
Noor Hanisa, Harun |
title |
Osteoblast Demineralization Induced by Oxidized High-Density
Lipoprotein via the Inflammatory Pathway Is Suppressed
by Adiponectin |
title_short |
Osteoblast Demineralization Induced by Oxidized High-Density
Lipoprotein via the Inflammatory Pathway Is Suppressed
by Adiponectin |
title_full |
Osteoblast Demineralization Induced by Oxidized High-Density
Lipoprotein via the Inflammatory Pathway Is Suppressed
by Adiponectin |
title_fullStr |
Osteoblast Demineralization Induced by Oxidized High-Density
Lipoprotein via the Inflammatory Pathway Is Suppressed
by Adiponectin |
title_full_unstemmed |
Osteoblast Demineralization Induced by Oxidized High-Density
Lipoprotein via the Inflammatory Pathway Is Suppressed
by Adiponectin |
title_sort |
osteoblast demineralization induced by oxidized high-density
lipoprotein via the inflammatory pathway is suppressed
by adiponectin |
publisher |
MDPI |
publishDate |
2022 |
url |
http://ir.unimas.my/id/eprint/40559/1/Osteoblast%20Demineralization%20-%20Copy.pdf http://ir.unimas.my/id/eprint/40559/ https://www.mdpi.com/1422-0067/23/23/14616/htm https://doi.org/10.3390/ijms232314616 |
_version_ |
1751540631320133632 |
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13.18916 |