Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5...
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Faculty of Food Science & Technology, UPM.
2009
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my.unimas.ir.400392022-10-04T00:11:42Z http://ir.unimas.my/id/eprint/40039/ Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia Zulkifli, Y. Alitheen, N.B. Son, Radu Raha, Abdul Rahim Samuel, Lihan Yeap, S.K. Nishibuchi, M. QR Microbiology In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic d dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus. Faculty of Food Science & Technology, UPM. 2009 Article PeerReviewed text en http://ir.unimas.my/id/eprint/40039/3/Random%20amplified%20-%20Copy.pdf Zulkifli, Y. and Alitheen, N.B. and Son, Radu and Raha, Abdul Rahim and Samuel, Lihan and Yeap, S.K. and Nishibuchi, M. (2009) Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. International Food Research Journal, 16. pp. 141-150. ISSN 2231 7546 http://www.ifrj.upm.edu.my/ |
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QR Microbiology Zulkifli, Y. Alitheen, N.B. Son, Radu Raha, Abdul Rahim Samuel, Lihan Yeap, S.K. Nishibuchi, M. Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
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In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic d dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into
4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus. |
format |
Article |
author |
Zulkifli, Y. Alitheen, N.B. Son, Radu Raha, Abdul Rahim Samuel, Lihan Yeap, S.K. Nishibuchi, M. |
author_facet |
Zulkifli, Y. Alitheen, N.B. Son, Radu Raha, Abdul Rahim Samuel, Lihan Yeap, S.K. Nishibuchi, M. |
author_sort |
Zulkifli, Y. |
title |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_short |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_full |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_fullStr |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_full_unstemmed |
Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
title_sort |
random amplified polymorphic dna-pcr and eric pcr analysis on
vibrio parahaemolyticus isolated from cockles in padang, indonesia |
publisher |
Faculty of Food Science & Technology, UPM. |
publishDate |
2009 |
url |
http://ir.unimas.my/id/eprint/40039/3/Random%20amplified%20-%20Copy.pdf http://ir.unimas.my/id/eprint/40039/ http://www.ifrj.upm.edu.my/ |
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1746213627596111872 |
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13.18916 |