Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring

• Main Author: Sharifah Nurain, Hussain
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
• Subjects: S Agriculture (General)
• Online Access: http://ir.unimas.my/id/eprint/39899/1/SHARIFAH%20NURAIN%20BINTI%20HUSSAIN%2024pgs.pdf; http://ir.unimas.my/id/eprint/39899/4/Sharifah%20Nurain%20ft.pdf; http://ir.unimas.my/id/eprint/39899/

Bacteria living in the soil are capable of synthesizing different type of enzymes that are vital in many biochemical processes. Amylase, known as starch hydrolysing enzyme, is one of indispensable enzyme which can be naturally produced by many bacterial species. The discoveries of the amylolytic microbial species will help to boost the advancement of enzyme exploitation in many biotechnological based industries especially in Sarawak. Thus, the aim of this research is to isolate the pure microbial strain from the soil sample at selected site of Panchor Hot Spring and screen for the potential amylase activity. In this study, three pure isolates were successfully isolated using nutrient agar and all tested isolates showed amylase activity when screened with starch hydrolysis assay. The rate of amylase activity of amylolytic strains was analysed by using 3,5- dinitrosalicylic (DNS) assay resulting in the highest activity by E1 isolate which was 143.85 U/ml. In conclusion, amylase producing bacteria was successfully isolated from soil sample of Panchor Hot Spring, however its enzymatic potential must be further studied before it can further be utilised for many industrial applications.