Optimisation of Solid-State Fermentation Parameters for the Xylanase Produced by Aspergillus niger using rice straw as the substrate

• Main Author: Nur Aidil Hakimy, Awang Ngah
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
• Subjects: QK Botany
• Online Access: http://ir.unimas.my/id/eprint/39873/1/NUR%20AIDIL%20HAKIMY%20BIN%20AWANG%20NGAH%2024pgs.pdf; http://ir.unimas.my/id/eprint/39873/2/NUR%20AIDIL%20HAKIMY%20BIN%20AWANG%20NGAH%20ft.pdf; http://ir.unimas.my/id/eprint/39873/

Rice straw is one of the most abundant lignocellulosic waste materials and a major agricultural by-product in Asia. Improper management of this agro-waste such as open field burning waste may become a serious threat to environment and health. Rice straw has been considered as a feasible medium for xylanase production under solid-state fermentation. This study focused on the optimization of SSF parameters for production of xylanase by Aspergillus niger utilizing rice straw as the substrate. The SSF parameters that were studied are initial moisture content of substrate, incubation temperature and period . Total enzyme activity was determined and amount of reducing sugar released was assessed through dinitrosalicylic acid method. The highest xylanase activity of 2.5352 ± 0.2667 U/ml was achieved at 70% (v/w) initial moisture content, 30°C of incubation temperature, and 4 days of incubation time.