A Possible Wound Convalescence of Frog Skin Peptide

• Main Author: Bong, Angeline Yee Chen
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/39608/1/Angeline%20Bong%20%20%20ft.pdf; http://ir.unimas.my/id/eprint/39608/

Antimicrobial peptides (AMPs) are a viable alternative to antimicrobial medicines for treating wounds. This study's objective was to identify potential wound convalescence peptides in the skin secretions of Pulchrana baramica and Hylarana erythraea at Universiti Malaysia Sarawak. The skin secretions of frogs captured in the vicinity of UNIMAS were partially purified using Sep-Pak six series cartridges. The partially purified peptides were then examined by SDS-PAGE to determine the amount of protein present in the samples. There were protein bands present in both species. P. baramica had protein bands of around 1.0 kDa. H. erythraea had protein bands of 1.0 kDa, 3.35 kDa, and 14.2 kDa. The antibacterial properties of partly purified peptides against P. baramica and H. erythraea were evaluated using a disc diffusion experiment, and the inhibition zone of the peptides was determined using a ruler. E. coli and S. aureus were used to inoculate the agar plate, which was then incubated with a filter paper disc containing the sample peptide. Taken was the average inhibition zone of the peptides. The partially purified peptides for H. erythraea contained an inhibition zone at a concentration of 500 g/mL, whereas the partially purified peptides for P. baramica lacked an inhibition zone. This study demonstrates the presence of potential AMPs in P. baramica and H. erythraea, and the disc diffusion assay supports the AMPs' antibacterial activity as wound convalescence peptides. This study will hopefully contribute to the current understanding of AMPs in frog skin secretion and aid in the development of alternatives to antibiotics.