Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli o157 :H7

• Main Authors: Wai Ling, Ooi, Son, Radu, Gulam, Rusul, Mohamed Ismail, Abdul Karim, Endang, Purwati, Samuel, Lihan
• Format: Article
• Language: English
• Published: Capricon Publication 2000
• Subjects: QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/39297/2/Enterobacterial%20-%20Copy.PDF; http://ir.unimas.my/id/eprint/39297/; https://scialert.net/abstract/?doi=pjbs.2000.35.37

A total of 30 strains of Escherichia coli 0157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus(ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.