Characterization of Vibrio vulni¢cus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid pro¢les and random ampli¢cation of polymorphic DNA analysis

• Main Authors: Son, Radu, Nasreldin, Elhadi, Zaiton, Hassan, Gulam, Rusul, Samuel, Lihan, Nimita, Fifadara, Yuherman, Y, Endang, Purwati
• Format: Article
• Language: English
• Published: Elsevier Science, Ltd. 1998
• Subjects: Q Science (General); QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/39245/3/Characterization%20of%20Vibrio%20-%20Copy.pdf; http://ir.unimas.my/id/eprint/39245/; https://www.sciencedirect.com/journal/fems-microbiology-letters; https://doi.org/10.1111/j.1574-6968.1998.tb13138.x

Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biotype 1 and two strains of biotype 2 contained plasmid DNA bands ranging in size from 1.4 to 9.7 MDa. Thirty-one (83.3%) were found to be resistant to one or more of the antimicrobial agents tested, however no specific correlation between antimicrobial resistance patterns and a single biotype was found. In addition, no particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers produced polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotype 1 and biotype 2 of the V. vulnificus strains investigated. RAPD identity across biotypes was also observed among Vibrio vulnificus strains.