Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish

The purpose of this study was to determine the prevalence pathogenic bacteria from various species of farmed freshwater fish and their antibiotic resistance patterns. Identification of the bacterial isolates was performed by GTG-PCR pattern analysis followed by 16S rRNA PCR sequencing. The antibioti...

Full description

Saved in:
Bibliographic Details
Main Authors: Sia, Siew Chuang, Samuel, Lihan, Tan, Cheng Siang, Chung, Hung Hui
Format: Proceeding
Language:English
Published: 2022
Subjects:
Online Access:http://ir.unimas.my/id/eprint/38782/3/Characterizations%20of%20bacterial.pdf
http://ir.unimas.my/id/eprint/38782/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.38782
record_format eprints
spelling my.unimas.ir.387822023-03-30T03:17:54Z http://ir.unimas.my/id/eprint/38782/ Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish Sia, Siew Chuang Samuel, Lihan Tan, Cheng Siang Chung, Hung Hui QR Microbiology The purpose of this study was to determine the prevalence pathogenic bacteria from various species of farmed freshwater fish and their antibiotic resistance patterns. Identification of the bacterial isolates was performed by GTG-PCR pattern analysis followed by 16S rRNA PCR sequencing. The antibiotic susceptibility test was performed by using the Kirby Bauer Disk Diffusion Method. In this study, the bacteria was randomly isolated from internal organ (liver, heart, kidney, internal organ fluid, eyeball fluid, and bile salt) of red tilapia (Oreochromis spp.), lampam (Puntius gonionotus), sultan fish (Leptobarbus hoevenii), patin (Pangasius spp.), and empurau (Tor tambroides). The bacteria acquired from the fishes were Aeromonas spp., Pseudomonas, Enterobacter, Citrobacter, Klebsiella, Plesiomonas, Enterococcus, and others. A total of 21 types of antibiotic were used in the susceptibility test and almost all of the bacteria strains were found to be resistant to two or three types of the antibiotics tested. The highest resistance was observed against Meropenem, Ertapenem, Ticarcillin and Imipenem among the tested bacteria. This indirectly showed the emerging multidrug resistant bacteria present in aquaculture of Borneo. In the future percept, the rapid diagnostic and treatment are essential as the view of the presence of the emerging multiantibiotic resistance bacteria. 2022-06 Proceeding PeerReviewed text en http://ir.unimas.my/id/eprint/38782/3/Characterizations%20of%20bacterial.pdf Sia, Siew Chuang and Samuel, Lihan and Tan, Cheng Siang and Chung, Hung Hui (2022) Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish. In: 4th National Seminar on Security and Sustainability Biology 2022 (4th Bioses 2022), 28-29th June 2022, Universiti Malaysia Terengganu.
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Sia, Siew Chuang
Samuel, Lihan
Tan, Cheng Siang
Chung, Hung Hui
Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish
description The purpose of this study was to determine the prevalence pathogenic bacteria from various species of farmed freshwater fish and their antibiotic resistance patterns. Identification of the bacterial isolates was performed by GTG-PCR pattern analysis followed by 16S rRNA PCR sequencing. The antibiotic susceptibility test was performed by using the Kirby Bauer Disk Diffusion Method. In this study, the bacteria was randomly isolated from internal organ (liver, heart, kidney, internal organ fluid, eyeball fluid, and bile salt) of red tilapia (Oreochromis spp.), lampam (Puntius gonionotus), sultan fish (Leptobarbus hoevenii), patin (Pangasius spp.), and empurau (Tor tambroides). The bacteria acquired from the fishes were Aeromonas spp., Pseudomonas, Enterobacter, Citrobacter, Klebsiella, Plesiomonas, Enterococcus, and others. A total of 21 types of antibiotic were used in the susceptibility test and almost all of the bacteria strains were found to be resistant to two or three types of the antibiotics tested. The highest resistance was observed against Meropenem, Ertapenem, Ticarcillin and Imipenem among the tested bacteria. This indirectly showed the emerging multidrug resistant bacteria present in aquaculture of Borneo. In the future percept, the rapid diagnostic and treatment are essential as the view of the presence of the emerging multiantibiotic resistance bacteria.
format Proceeding
author Sia, Siew Chuang
Samuel, Lihan
Tan, Cheng Siang
Chung, Hung Hui
author_facet Sia, Siew Chuang
Samuel, Lihan
Tan, Cheng Siang
Chung, Hung Hui
author_sort Sia, Siew Chuang
title Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish
title_short Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish
title_full Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish
title_fullStr Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish
title_full_unstemmed Characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish
title_sort characterizations of bacterial pathogens isolated from internal organ of infected farmed freshwater fish
publishDate 2022
url http://ir.unimas.my/id/eprint/38782/3/Characterizations%20of%20bacterial.pdf
http://ir.unimas.my/id/eprint/38782/
_version_ 1762396702968381440
score 13.222552