Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan)

• Main Author: Chia, Chun Min
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
• Subjects: QK Botany
• Online Access: http://ir.unimas.my/id/eprint/36411/1/CHIA%20CHUN%20MIN%20%2824%20pgs%29.pdf; http://ir.unimas.my/id/eprint/36411/4/CHIA%20CHUN%20ft.pdf; http://ir.unimas.my/id/eprint/36411/

Overpopulation and increase in demand of timer have caused the depletion of timber resources. Therefore, Neolamarckia macrophylla (red kelampayan) belongs to family Rubiaceae is selected as one of the species for forest plantation and reforestation programmes in Sarawak due to its fast growing characteristic. However, conventional propagation of N. macrophylla is time consuming and possessed several problems mentioned. Therefore, in vitro shoot induction of N. macrophylla should be explored for mass propagation purposes in order to meet the global demands for timber resources. Effect of BAP on in vitro shoot induction of N. macrophylla was investigated. The optimum concentration of BAP on in vitro shoot induction of N. macrophylla was determined. In this study, B5 media supplemented with different concentration of BAP (0.5, 1.0 and 1.5 mg/L) was used for in vitro shoot induction from nodal explants of N. macrophylla. The parameters used in this study were number of shoots, shoot regeneration percentage (%), multiple shoot induction percentage (%) and shoot length (cm). The data was analyzed by using One-way ANOVA and Duncan's Multiple Range Test (DMRT) in SPSSS version 22. Result showed that the B5 supplemented with 0.5 mg/L BAP had given the highest mean number of shoots with 3.80 shoots per explant. 100% of shoot regeneration was observed in B5 treated with 0.5 and 1.0 mg/L while highest shoot length was observed in B5 blank. One-way ANOVA showed significant effect of BAP in terms of number of shoots regenerated. In this study, B5 supplemented with 0.5 mg/L BAP was identified as optimum concentration on in vitro shoot induction of N. macrophylla.