Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis
Chromosome count is the only direct way to determine the number of chromosomes of a species. This study is often considered trivial that seldom described and discussed in detail. Therefore, it is inevitable that the chromosome count protocol should be revised and revisited before it becomes oblitera...
Saved in:
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Horticulture and Forestry Society from Transylvania
2021
|
Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/36212/1/chromosome1.pdf http://ir.unimas.my/id/eprint/36212/ https://www.notulaebiologicae.ro/index.php/nsb/article/view/10995 https://doi.org/10.15835/nsb13310995 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.unimas.ir.36212 |
---|---|
record_format |
eprints |
spelling |
my.unimas.ir.362122021-09-28T02:56:53Z http://ir.unimas.my/id/eprint/36212/ Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis Wee Hiang, Eng Wei Seng, Ho Kwong-Hung, LING SB Plant culture SD Forestry Chromosome count is the only direct way to determine the number of chromosomes of a species. This study is often considered trivial that seldom described and discussed in detail. Therefore, it is inevitable that the chromosome count protocol should be revised and revisited before it becomes obliterated. In the present study, we encountered challenges in obtaining a clear micrograph for the chromosome count of active mitotic cells of Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) root tips. Several obstacles were determined through micrograph observation, such as existing unwanted particles in cells, poor chromosome staining and chromosome clumping. To overcome these, root tip types, staining methodologies, squashing methods were among the factors assessed to obtain clear micrographs. The chromosome counts of N. cadamba under optimized procedure showed 2n = 44 chromosomes. We also apply digital technology in chromosome counts, such as online databases and graphic software that are open source and freely accessible to the public. Only basic laboratory equipment and chemicals were used throughout the study, thus making this study economical and applicable in a basic laboratory. The availability of online digital software and databases provide open-source platforms that will ease the efforts in chromosome count. Horticulture and Forestry Society from Transylvania 2021-08-18 Article PeerReviewed text en http://ir.unimas.my/id/eprint/36212/1/chromosome1.pdf Wee Hiang, Eng and Wei Seng, Ho and Kwong-Hung, LING (2021) Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis. Notulae Scientia Biologicae, 13 (3). pp. 1-14. ISSN 2067-3264 https://www.notulaebiologicae.ro/index.php/nsb/article/view/10995 https://doi.org/10.15835/nsb13310995 |
institution |
Universiti Malaysia Sarawak |
building |
Centre for Academic Information Services (CAIS) |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaysia Sarawak |
content_source |
UNIMAS Institutional Repository |
url_provider |
http://ir.unimas.my/ |
language |
English |
topic |
SB Plant culture SD Forestry |
spellingShingle |
SB Plant culture SD Forestry Wee Hiang, Eng Wei Seng, Ho Kwong-Hung, LING Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
description |
Chromosome count is the only direct way to determine the number of chromosomes of a species. This study is often considered trivial that seldom described and discussed in detail. Therefore, it is inevitable that the chromosome count protocol should be revised and revisited before it becomes obliterated. In the present study, we encountered challenges in obtaining a clear micrograph for the chromosome count of active mitotic cells of Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) root tips. Several obstacles were determined through micrograph observation, such as existing unwanted particles in cells, poor chromosome staining and chromosome clumping. To overcome these, root tip types, staining methodologies, squashing methods were among the factors assessed to obtain clear micrographs. The chromosome counts of N. cadamba under optimized procedure showed 2n = 44 chromosomes. We also apply digital technology in chromosome counts, such as online databases and graphic software that are open source and freely accessible to the public. Only basic laboratory equipment and chemicals were used throughout the study, thus making this study economical and applicable in a basic laboratory. The availability of online digital software and databases provide open-source platforms that will ease the efforts in chromosome count. |
format |
Article |
author |
Wee Hiang, Eng Wei Seng, Ho Kwong-Hung, LING |
author_facet |
Wee Hiang, Eng Wei Seng, Ho Kwong-Hung, LING |
author_sort |
Wee Hiang, Eng |
title |
Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
title_short |
Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
title_full |
Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
title_fullStr |
Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
title_full_unstemmed |
Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
title_sort |
cytogenetic, chromosome count optimization and automation of neolamarckia cadamba (rubiaceae) root tips derived from in vitro mutagenesis |
publisher |
Horticulture and Forestry Society from Transylvania |
publishDate |
2021 |
url |
http://ir.unimas.my/id/eprint/36212/1/chromosome1.pdf http://ir.unimas.my/id/eprint/36212/ https://www.notulaebiologicae.ro/index.php/nsb/article/view/10995 https://doi.org/10.15835/nsb13310995 |
_version_ |
1712289139488980992 |
score |
13.18916 |