Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’
Studies of Fortunella polyandra ‘Nagami’ and ‘Meiwa’ were carried out to observe their responses in tissue culture systems. Explant sources investigated included roots, stems, leaves and cotyledons which were cultured on Murashige and Skoog (MS) medium supplemented with different combinations and...
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my.unimas.ir.362092021-09-28T02:39:08Z http://ir.unimas.my/id/eprint/36209/ Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’ N., Mohamed R.M., Taha Hashimah, Elias S., Abdullah N.A., Hasbullah S Agriculture (General) SB Plant culture Studies of Fortunella polyandra ‘Nagami’ and ‘Meiwa’ were carried out to observe their responses in tissue culture systems. Explant sources investigated included roots, stems, leaves and cotyledons which were cultured on Murashige and Skoog (MS) medium supplemented with different combinations and concentrations of various hormones. The hormones used were naphthalene acetic acid (NAA) and benzylaminopurine (BAP) at pH 5.8 with a temperature of 23-26°C and a photoperiod of 16 hours light and 8 hours dark. The pH of the media was also altered to include 4.8, 5.8, 6.8 and 7.8. The various explants were subjected to light and dark treatments in order to study the morphogenesis of this species. From the results, it was observed that stem and leaf explants were more responsive than other explants. The best media for regeneration and callus formation was MS supplemented with 0.5 mg/L NAA and 0.5 mg/L BAP at pH 5.8 for ‘Nagami’ and pH 7.8 for ‘Meiwa’. Regeneration was achieved via direct organogenesis and also via callus formation. The highest percentage of shoot formation (40%) was obtained from stem explants (‘Meiwa’) cultured on MS supplemented with 0.5 mg/L NAA and 0.5 mg/L BAP at pH 7.8. ISHS 2013 Article PeerReviewed text en http://ir.unimas.my/id/eprint/36209/1/polyandra1.pdf N., Mohamed and R.M., Taha and Hashimah, Elias and S., Abdullah and N.A., Hasbullah (2013) Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’. Acta Horticulturae, 975 (38). pp. 315-322. ISSN 0567-7572 (print) 2406-6168 (electronic) https://www.actahort.org/books/975/975_38.htm 10.17660/ActaHortic.2013.975.38 |
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S Agriculture (General) SB Plant culture N., Mohamed R.M., Taha Hashimah, Elias S., Abdullah N.A., Hasbullah Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’ |
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Studies of Fortunella polyandra ‘Nagami’ and ‘Meiwa’ were carried out to
observe their responses in tissue culture systems. Explant sources investigated
included roots, stems, leaves and cotyledons which were cultured on Murashige and
Skoog (MS) medium supplemented with different combinations and concentrations of
various hormones. The hormones used were naphthalene acetic acid (NAA) and
benzylaminopurine (BAP) at pH 5.8 with a temperature of 23-26°C and a photoperiod
of 16 hours light and 8 hours dark. The pH of the media was also altered to include
4.8, 5.8, 6.8 and 7.8. The various explants were subjected to light and dark treatments
in order to study the morphogenesis of this species. From the results, it was observed
that stem and leaf explants were more responsive than other explants. The best media
for regeneration and callus formation was MS supplemented with 0.5 mg/L NAA and
0.5 mg/L BAP at pH 5.8 for ‘Nagami’ and pH 7.8 for ‘Meiwa’. Regeneration was
achieved via direct organogenesis and also via callus formation. The highest
percentage of shoot formation (40%) was obtained from stem explants (‘Meiwa’)
cultured on MS supplemented with 0.5 mg/L NAA and 0.5 mg/L BAP at pH 7.8. |
format |
Article |
author |
N., Mohamed R.M., Taha Hashimah, Elias S., Abdullah N.A., Hasbullah |
author_facet |
N., Mohamed R.M., Taha Hashimah, Elias S., Abdullah N.A., Hasbullah |
author_sort |
N., Mohamed |
title |
Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’ |
title_short |
Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’ |
title_full |
Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’ |
title_fullStr |
Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’ |
title_full_unstemmed |
Tissue Culture Studies on Fortunella polyandra ‘Nagami’ and ‘Meiwa’ |
title_sort |
tissue culture studies on fortunella polyandra ‘nagami’ and ‘meiwa’ |
publisher |
ISHS |
publishDate |
2013 |
url |
http://ir.unimas.my/id/eprint/36209/1/polyandra1.pdf http://ir.unimas.my/id/eprint/36209/ https://www.actahort.org/books/975/975_38.htm |
_version_ |
1712289139029704704 |
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13.18916 |