A revisit to a low-cost method for the isolation of microsatellite markers: the case of the endangered Malayan tapir (Tapirus indicus)

• Main Authors: Qi Luan, Lim, Nurul Adilah, Ismail, Ramitha, Arumugam, Wei Lun, Ng, Christina Seok Yien, Yong, Ahmad, Ismail, Jeffrine J., Rovie-Ryan, Norsyamimi, Rosli, Geetha, Annavi
• Format: Article
• Language: English
• Published: Malaysian Nature Society 2019
• Subjects: QL Zoology
• Online Access: http://ir.unimas.my/id/eprint/35979/1/tapir1.pdf; http://ir.unimas.my/id/eprint/35979/; https://doi.org/10.1101/384651

There are many approaches to develop microsatellite markers. Despite the availability of the more advanced technology in the market, due to budget constraints, we revisited an easy and rapid polymerase chain reaction (PCR) cloning-sequencing method to design microsatellite markers for Tapirus indicu...