Protein profiling of mutant type and wild type of Leucaena leucocephala
Leucaena leucocephala is also known as Petai Belalang tree. This leaf of L. leucocephala has many benefits toward human and animals such as fodder, fuel and charcoal. The proteins were extracted by using the three different methods; phenol, TCA and imidazole for mutant and wild type of L. leucoceph...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2018
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/35767/1/AMIRAH%20FASYA%20%2824%20pgs%29.pdf http://ir.unimas.my/id/eprint/35767/4/Amirah%20Fasya%20Binti%20Jama%20ft.pdf http://ir.unimas.my/id/eprint/35767/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Leucaena leucocephala is also known as Petai Belalang tree. This leaf of L. leucocephala has many benefits toward human and animals such as fodder, fuel and charcoal. The proteins
were extracted by using the three different methods; phenol, TCA and imidazole for mutant and wild type of L. leucocephala. The extraction methods have different substances in the
extraction buffer that can give the process of extraction become more efficient. The purpose of this study is to identify the most suitable methods for extraction the proteins between
phenol, TCA and imidazole methods by observing the band intensity on the gel of SDS-PAGE. TCA method has shown the clear and bright bands for wild type and mutant of
L. leucocephala. Therefore, the TCA is the most suitable method method among phenol and imidazole has proceeded into 2-D PAGE whereby there is difference in spot intensity for wild
type is 0.557 while mutant is 0.363 by using Delta2D software. |
|---|