Comparative Proteome of Irradiated Mutant and Wild Type Oryza Sativa

• Main Author: Joyce Grace, John
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2018
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/35387/1/Joyce.pdf; http://ir.unimas.my/id/eprint/35387/4/Joyce%20full.pdf; http://ir.unimas.my/id/eprint/35387/

In this research, the comparative proteome is the protein analysis of irradiated mutant and wild type Oryza sativa to detect the presence or absence of proteins and the direct measurement of relative protein abundances. This is done first by the extraction of total protein from the sample, and then protein was separated by using SDS - PAGE and 2D - PAGE. These proteomic methods were used to obtain a protein profile ofrice total seed proteins and identify important functional protein component. Then, results were analyzed using Delta2D - Gel analysis software. Overall, matching protein spots of irradiated mutant varieties of Mutant 28, Mutant 39 and Mutant 40 with variety MR219 - wild type were 101, 103 and 107 respectively. These results expand our understanding of the rice proteome and improve our knowledge of the cellular biology of rice seeds. To obtain the new desirable trait in crops, mutation breeding was vastly used. Since, mutation cause changes in protein expression, protein analysis was done to know which protein undergo alteration.