The Potential Use of Cassia alata Linn. Extracts to Inhibit Cellulitis Causeing Agent
Cassia ala ta Linn. ( C. ala ta Linn.) is a shrub believed to have antimicrobial activity towards certain bacteria including Staphylococcus aureus (S. aureus). Thus, the flower extracts of C. alata Linn. were investigated for their antimicrobial activity on the strains of S. aureus. The extraction...
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2018
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| Online Access: | http://ir.unimas.my/id/eprint/35386/1/The%20Potential%20Use%20of%20Cassia%20alata%20Linn.%20Extracts%20to%20Inhibit%20Cellulitis%20Causeing%20Agent%2824pgs%29.pdf http://ir.unimas.my/id/eprint/35386/4/Gedlyn%20Steffi.pdf http://ir.unimas.my/id/eprint/35386/ |
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| Summary: | Cassia ala ta Linn. ( C. ala ta Linn.) is a shrub believed to have antimicrobial activity towards certain bacteria
including Staphylococcus aureus (S. aureus). Thus, the flower extracts of C. alata Linn. were investigated for
their antimicrobial activity on the strains of S. aureus. The extraction of the flower was done in four solvents
which were n-Hexane, ethyl-acetate, sterile distilled water and ethanol. After the extract was diluted to the
concentration of IO µg/µI, it was shown to best inhibit the growth of S. aureus for the solvent ethyl-acetate and
n-Hexane. That shows a higher concentration is the best condition to exhibit the antimicrobial properties for C.
alata Linn. extract. The antimicrobial testing were carried out using well diffusion, minimum inhibitory
concentration (MIC) and time kill assay. The extracts used were dissolved in four solvents that were n-Hexane,
ethanol, ethyl acetate and sterile distilled water. The extracts were extracted using maceration and Soxhlet
techniques. The MIC values obtained for solvents extracted using maceration technique were 1200 µg/µL for nHexane and ethanol while 300 µg/µL for ethyl acetate. There were no inhibitions in sterile distilled water. The
MIC value for n-Hexane and ethyl acetate extracted using Soxhlet technique was 600 µg/µL and 2400 µg/µL for
ethanol and distilled water, respectively. Therefore, there is the potential use of Cassia alata Linn. extracts to
inhibit cellulitis causing agent S. aureus |
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