Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish
ABCB5 (ATP-binding cassette, subfamily B, member 5) protein consists of P-glycoprotein that affects the levels of plasma and intracellular drugs concentration that control therapeutic response. This study aims to isolate and characterize the promoter region of the ABCB5 gene from zebrafish (Danio...
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Universiti Malaysia Sarawak (UNIMAS)
2018
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my.unimas.ir.353842023-10-19T09:09:07Z http://ir.unimas.my/id/eprint/35384/ Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish Auni Haziqah, Sama Jamal Q Science (General) ABCB5 (ATP-binding cassette, subfamily B, member 5) protein consists of P-glycoprotein that affects the levels of plasma and intracellular drugs concentration that control therapeutic response. This study aims to isolate and characterize the promoter region of the ABCB5 gene from zebrafish (Danio rerio). Initially, DNA extraction was performed on zebrafish by using lysis buffer. Then, the primer for the ABCB5 promoter was designed and the gradient PCR was done with the optimum temperature 53.5°C to produce single amplicon with the approximate size of 1,500 bp. The amplicon was cloned into pGEM-T Easy Vector and transformed into Escherichia coli XLl Blue Competent cells with the transformation efficiency of 0.05x10° transform ants per µg. Colony PCR was conducted to determine the positive colony with promoter insert. Next, plasmid miniprep was conducted before sending the plasmid for sequencing. However, the BLASTn results showed that the sequence cloned is not ABCB5 promoter region from zebrafish. Hence, it is further suggested that cloning should be continued in order to determine the transcription factor which regulates ABCB5 in order for us to further und Universiti Malaysia Sarawak (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35384/1/Auni%20Haziqah%20%20ft.pdf Auni Haziqah, Sama Jamal (2018) Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish. [Final Year Project Report] (Unpublished) |
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Q Science (General) Auni Haziqah, Sama Jamal Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish |
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ABCB5 (ATP-binding cassette, subfamily B, member 5) protein consists of P-glycoprotein
that affects the levels of plasma and intracellular drugs concentration that control
therapeutic response. This study aims to isolate and characterize the promoter region of the
ABCB5 gene from zebrafish (Danio rerio). Initially, DNA extraction was performed on
zebrafish by using lysis buffer. Then, the primer for the ABCB5 promoter was designed
and the gradient PCR was done with the optimum temperature 53.5°C to produce single
amplicon with the approximate size of 1,500 bp. The amplicon was cloned into pGEM-T
Easy Vector and transformed into Escherichia coli XLl Blue Competent cells with the
transformation efficiency of 0.05x10° transform ants per µg. Colony PCR was conducted to
determine the positive colony with promoter insert. Next, plasmid miniprep was conducted
before sending the plasmid for sequencing. However, the BLASTn results showed that the
sequence cloned is not ABCB5 promoter region from zebrafish. Hence, it is further
suggested that cloning should be continued in order to determine the transcription factor
which regulates ABCB5 in order for us to further und |
format |
Final Year Project Report |
author |
Auni Haziqah, Sama Jamal |
author_facet |
Auni Haziqah, Sama Jamal |
author_sort |
Auni Haziqah, Sama Jamal |
title |
Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish |
title_short |
Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish |
title_full |
Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish |
title_fullStr |
Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish |
title_full_unstemmed |
Isolation and Characterization of ABCB5 Gene Promoter Region from Zebrafish |
title_sort |
isolation and characterization of abcb5 gene promoter region from zebrafish |
publisher |
Universiti Malaysia Sarawak (UNIMAS) |
publishDate |
2018 |
url |
http://ir.unimas.my/id/eprint/35384/1/Auni%20Haziqah%20%20ft.pdf http://ir.unimas.my/id/eprint/35384/ |
_version_ |
1781710318076952576 |
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13.189131 |