Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3)
The heterologous expression of cellulase gene from Aspergillus sp. in E.coli strains of Rosetta (DE3) and BL2 l (DE3) has been studied to compare the recombinant protein production and to observe their activity, efficiency and production rate of recombinant cellulase. In previous study, an improv...
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Universiti Malaysia Sarawak, (UNIMAS)
2018
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my.unimas.ir.353792023-03-02T08:43:15Z http://ir.unimas.my/id/eprint/35379/ Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3) Casandra, Kana Brooke Q Science (General) The heterologous expression of cellulase gene from Aspergillus sp. in E.coli strains of Rosetta (DE3) and BL2 l (DE3) has been studied to compare the recombinant protein production and to observe their activity, efficiency and production rate of recombinant cellulase. In previous study, an improved protein quality has been detected by using Rosetta, therefore further works need to be carried out to compare its protein production with BL2 l. The pFT AG-vector with the recombinant cellulase was used for expression studies and inserted into both Rosetta and BL2 l. Screening of cellullolyctic organism has failed to reveal the activity of recombinant cellulase as there is no growth detected on the media, as well as no formation of halo zone. Other than detection using the formation of halo zone, Bradford Assay and DNS assay was conducted to determine the cellulase activity of the bacterial in liquid medium. Recombinant cellulase from Rosetta was shown to have higher cellulase activity when the protein concentration was calculated using the standard graph. It was due to the fact that Rosetta has rare codons implemented inside them in order to yield higher protein production and better protein purity Universiti Malaysia Sarawak, (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35379/1/Heterologous%20Expression%20Of%20Recombinant%20Cellulase%20In%20Escherichia%20Coli%20Rosetta%20%28De3%29%20And%20Bl21%20%28De3%29%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/35379/4/Heterologous%20Expression..ft.pdf Casandra, Kana Brooke (2018) Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3). [Final Year Project Report] (Unpublished) |
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Q Science (General) Casandra, Kana Brooke Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3) |
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The heterologous expression of cellulase gene from Aspergillus sp. in E.coli strains of Rosetta
(DE3) and BL2 l (DE3) has been studied to compare the recombinant protein production and to
observe their activity, efficiency and production rate of recombinant cellulase. In previous study,
an improved protein quality has been detected by using Rosetta, therefore further works need to
be carried out to compare its protein production with BL2 l. The pFT AG-vector with the
recombinant cellulase was used for expression studies and inserted into both Rosetta and BL2 l.
Screening of cellullolyctic organism has failed to reveal the activity of recombinant cellulase as
there is no growth detected on the media, as well as no formation of halo zone. Other than
detection using the formation of halo zone, Bradford Assay and DNS assay was conducted to
determine the cellulase activity of the bacterial in liquid medium. Recombinant cellulase from
Rosetta was shown to have higher cellulase activity when the protein concentration was
calculated using the standard graph. It was due to the fact that Rosetta has rare codons
implemented inside them in order to yield higher protein production and better protein purity |
format |
Final Year Project Report |
author |
Casandra, Kana Brooke |
author_facet |
Casandra, Kana Brooke |
author_sort |
Casandra, Kana Brooke |
title |
Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3) |
title_short |
Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3) |
title_full |
Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3) |
title_fullStr |
Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3) |
title_full_unstemmed |
Heterologous Expression Of Recombinant Cellulase In Escherichia Coli Rosetta (De3) And Bl21 (De3) |
title_sort |
heterologous expression of recombinant cellulase in escherichia coli rosetta (de3) and bl21 (de3) |
publisher |
Universiti Malaysia Sarawak, (UNIMAS) |
publishDate |
2018 |
url |
http://ir.unimas.my/id/eprint/35379/1/Heterologous%20Expression%20Of%20Recombinant%20Cellulase%20In%20Escherichia%20Coli%20Rosetta%20%28De3%29%20And%20Bl21%20%28De3%29%2824pgs%29.pdf http://ir.unimas.my/id/eprint/35379/4/Heterologous%20Expression..ft.pdf http://ir.unimas.my/id/eprint/35379/ |
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1759693322632298496 |
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13.15806 |