Isolation and Enumeration of Vibrio cholerae ctxA from Street Vendor Drink

• Main Author: Nur Sabrina, Habib
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/35378/1/Nur%20Sabrina.pdf; http://ir.unimas.my/id/eprint/35378/4/Nur%20Sabrina%20full.pdf; http://ir.unimas.my/id/eprint/35378/

Cholera remains a global threat to the public's health in Malaysia because the outbreaks occur sporadically. Knowledge on the rate of outbreak and pattern distribution is needed as well as its prevalence in food in order to control the foodborne outbreak of cholera. The study aims to isolate and enumerate Vibrio cholerae and to detect the presence of pathogenic or virulence strain of V. cholerae in drink samples from street vendor in Kuching. A total of 30 (n=30) drink samples was collected from two types of drinks, drinks containing milk and drinks without milk. MPN-Plate method was conducted to estimate the concentration of V. cholerae and PCR was done with the ctxA gene to verify the pathogenicity of V. cholerae. Im this project, it was shown that the occurrence of V. cholerae in drinks with milk is higher compared to drinks without milk. Drink samples with milk had an MPN/g estimation with a minimum value of <3 MPN/g and a maximum value of> 1 JOO MPN/g. Whereas, drink samples without milk had an MPN/g estimation with a minimum value of <3 MPN/g and a maximum value of 460 MPN/g. However, none of the isolates were found to contain the ctxA gene. This study could highlights the importance of public education in the proper handling of food and be adequately informed about potential microbiological hazards of food handling.