Isolation and Characterization of Vibrio cholerae from Water in Aquaculture Farms
Aquaculture is an important sector in agriculture that supply protein source. However, the presence of pathogenic bacteria in aquaculture products may cause serious health problems. Vibrio cholerae is one of pathogenic bacteria that can cause food borne disease. In this study, we aim to isolate Vi...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2018
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/35178/1/Laurie.pdf http://ir.unimas.my/id/eprint/35178/4/Laurie%20Ali%20ft.pdf http://ir.unimas.my/id/eprint/35178/ |
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| Summary: | Aquaculture is an important sector in agriculture that supply protein source. However, the presence of
pathogenic bacteria in aquaculture products may cause serious health problems. Vibrio cholerae is one of
pathogenic bacteria that can cause food borne disease. In this study, we aim to isolate Vibrio cholerae
isolates from water in commercial and traditional farms. Isolation was done using selective growth on
TCBS agar, morphological and three biochemical identifications. A total of 12 Vibrio cholerae isolates
were confirmed out of a total of 60 presumptive isolates. The isolates were later further confirmed using
specific PCR targeting ctxA gene to detect pathogenic strain. ctxA encodes for cholera toxin gene. All 12
isolates do not harbor ctxA genes. Antibiotic susceptibility test was also done on the 12 isolates and
recorded a high antibiotic resistance profile. This study revealed the antibiotic resistance pattern of Vibrio
cholerae towards antimicrobial agents. Constant monitoring on antibiotic resistance pattern of Vibrio
cholerae in aquaculture farms should be done to prevent future resistance problems. |
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