Growth Kinetics and Lipid Accumulation in Rhodotorula mucilaginosa MV-5 Cultured in Sago Effluent

• Main Author: Reynold, Ramsy
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2018
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/35177/1/Reynold%20anak%20Ramsy.pdf; http://ir.unimas.my/id/eprint/35177/4/Reynold%20%20Ramsy%20ft.pdf; http://ir.unimas.my/id/eprint/35177/

The increase in the world demand for diesel has led to the production ofbiodiesel from alternatives sources. One of the example of these alternative is from fungal lipids from oleaginous yeast such as Rhodotorula sp. Fungal lipid is used as feedstock to replace plant and animal oils as the latter are competitive in terms of food and feeds. In this study, the growth kinetics of Rhodotorula mucilaginosa MV-5 shows that the culture is at log phase after 24 h. The highest biomass recorded during propagation stage was 1.63 g/L at 24 h. Next, R. mucilaginosa MV-5 was supplied with sago effluent as carbon source and the highest lipid extracted during lipid accumulation stage was 0.23 g/L. However, percentage of lipid by weight was not able to be determine due to presence of sago hampas particle which interfere with the actual weight of biomass. Phenol-sulphuric assay was used to determine the amount of starch reduction and the amount of starch reduced over the 168 h was 0.659 g/L. In conclusion, Rhodotorula mucilaginosa MV-5 can utilise sago effluent as carbon source to produce lipid.