Testing and Application of Fast and Easy Fungal Identification (feFiD) Kit on Different Types of Macro and Micro Fungi Isolated from Field Work
Fast and Easy Fungal Identification (feFiD) kit is produced to make fungal identification are faster and easier than conventional fungal identification. This is due to the identification at the morphological alone are not enough in identification of fungal especially at the species level. The aim of...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2018
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| Online Access: | http://ir.unimas.my/id/eprint/35172/2/KHAIRUN.pdf http://ir.unimas.my/id/eprint/35172/ |
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| Summary: | Fast and Easy Fungal Identification (feFiD) kit is produced to make fungal identification are faster and easier than conventional fungal identification. This is due to the identification at the morphological alone are not enough in identification of fungal especially at the species level. The aim of the research is to test the
application of feFiD kit in identification of macro-fungi and micro-fungi isolated from the fieldworks and
determine the limitation of the kit on various types of macro and micro fungi. There are total of eight samples of macro-fungi, and seventeen' samples of micro-fungi had been successfully identified by using feFiD kit.
Identification of all samples was done by aligning the DNA sequences using BLAST analysis to search for
closed match sequence in GenBank database. Based on the result obtained, all the samples revealed to be from
three different phylum which are Basidiomycota with eight species identified, Ascomycota with sixteen species
and one species from Zygomycota make it twenty five different species in total. The identity score from BLAST
analysis for all samples were in range from 85%- 100% for all samples. |
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