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Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase

Amylase is an endo-type enzyme that offers attractive economic benefit in many industrial purpose. In this work, induction studies with recombinant E. coli BL21 expressing heterologous amylase was studied. The effect of different carbon sources and the cultivation temperature on E.coli BL21 expres...

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Bibliographic Details
Main Author: Emalina, Faysial
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2018
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/35169/1/Emalina%20Faysial%20ft.pdf
http://ir.unimas.my/id/eprint/35169/
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spelling my.unimas.ir.351692023-07-27T09:48:40Z http://ir.unimas.my/id/eprint/35169/ Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase Emalina, Faysial Q Science (General) Amylase is an endo-type enzyme that offers attractive economic benefit in many industrial purpose. In this work, induction studies with recombinant E. coli BL21 expressing heterologous amylase was studied. The effect of different carbon sources and the cultivation temperature on E.coli BL21 expressing amylase was investigated. The result from this study showed that glucose was found to be the best carbon source for high amylase activity. Besides that, glycerol could be used as an alternative due to its promising performance. Meanwhile, 37 °C was found to be the optimum temperature for expressing amylase in recombinant E. coli BL2 l. Further studies on the induction should be continued in future in order to support the current results. Universiti Malaysia Sarawak (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35169/1/Emalina%20Faysial%20ft.pdf Emalina, Faysial (2018) Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Emalina, Faysial
Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase
description Amylase is an endo-type enzyme that offers attractive economic benefit in many industrial purpose. In this work, induction studies with recombinant E. coli BL21 expressing heterologous amylase was studied. The effect of different carbon sources and the cultivation temperature on E.coli BL21 expressing amylase was investigated. The result from this study showed that glucose was found to be the best carbon source for high amylase activity. Besides that, glycerol could be used as an alternative due to its promising performance. Meanwhile, 37 °C was found to be the optimum temperature for expressing amylase in recombinant E. coli BL2 l. Further studies on the induction should be continued in future in order to support the current results.
format Final Year Project Report
author Emalina, Faysial
author_facet Emalina, Faysial
author_sort Emalina, Faysial
title Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase
title_short Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase
title_full Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase
title_fullStr Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase
title_full_unstemmed Induction Studies with Recombinant Escherichia coli BL21 Expressing Heterologous Amylase
title_sort induction studies with recombinant escherichia coli bl21 expressing heterologous amylase
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/35169/1/Emalina%20Faysial%20ft.pdf
http://ir.unimas.my/id/eprint/35169/
_version_ 1772816285068951552
score 13.160551

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